GBL® ROSA-STAIN

5074-Colloidal Iron Acid for Mucin

Title Description
REF No 5074
Purpose Used to demonstrate acid epithelial mucin (sialomucin, sulfomucin) and stromal (mesenchymal) mucin.
Ingredients A) Acetic Acid Aqueous Solution: Glacial Acetic Acid
B) Colloidal Iron Stock Solution: Iron (III) Chloride
C) Acetic Acid, Glacial: Glacial Acetic Acid
D) Potassium Ferrocyanide Solution: Potassium Ferrocyanide Trihydrate
E) Hydrochloric Acid Solution: 37% Hydrochloric Acid
F) Van Gieson Solution: Picric Acid, Acid Fuchsin
Working Principle Colloidal Iron Acid; Mucin Staining is a technique used to histologically identify mucus and acidic mucopolysaccharides (mucin). This method is an important tool for identifying and examining mucus-containing cells and tissues. Colloidal iron staining is based on the binding of acidic mucopolysaccharides and mucus to iron ions. Once bound to acidic mucopolysaccharides, iron is visualized by a Prussian blue reaction.
Warnings, Precautions, Recommendations Danger. Flammable liquid and vapour. May cause cancer. Causes severe skin burns and eye damage. Wear protective gloves/protective clothing/eye protection/face protection. IF exposed or concerned: get medical advice/attention. IF SWALLOWED: rinse mouth. DO NOT induce vomiting. IF ON SKIN (or hair): remove/take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Causes skin irritation.
Application Pre-Staining Preparation:
1. It is recommended to use clean glassware in the staining procedure.

2. Preparation of Colloidal Iron Working Solution: Add and mix.
a. B-Colloidal Iron Stock Solution 20 ml
b. C-Acetic Acid, Glacial 5 ml
c. Distilled Water 15 ml

Staining:
3. Deparaffinize the sections by placing them in xylene for 3 minutes. Repeat this process 3 times. Then dip them in 100% and 96% denatured ethyl alcohol twice. Wash thoroughly with distilled water.
4. Place in Solution A (Acetic Acid Solution, 12%) for 30 seconds.
5. Dry the slides. Do not wash.
6. Place in Colloidal Iron Working Solution for 30 minutes.
7. Wash 3 times with Solution A (Acetic Acid Solution, 12%) for 3 minutes each.

8. Prepare Ferrocyanide-Hydrochloric Acid Solution just before use. Add and mix well.
a. Solution D: Potassium Ferrocyanide Solution, 2% 20 ml
b. Solution E: Hydrochloric Acid Solution, 2% 20 ml

9. Place in Ferrocyanide-Hydrochloric Acid Solution for 15 minutes.
10. Wash in tap water for 5 minutes.
11. Counterstain in Solution F: Van Gieson Stain for 3-5 minutes.

Continue directly to dehydration step without rinsing.
12. Dip twice in 100% denatured ethyl alcohol, dry. Then dip the slide in xylene three times and cover the slide.
Theoretical Results
Acid Epithelial Mucin Blue
Stromal Mucin Blue
Cryptococcus neoformans capsule Blue
Collagen Red
Muscle and cytoplasm Yellow

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15-25 °C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
445074050050 Piece 50 mL 50 ml, White Bottle with HDPE Dropper 1 piece
Piece 250 mL 250 ml, PP White Bottle with Dropper 1 piece
Piece 125 mL 125 ml, PP White Bottle with Dropper 3 piece
Piece 500 mL 500 ml, HDPE White Bottle 28 mm 2 piece

Keywords Colloidal Iron Acid; Mucin, Colloidal Iron Stain, Colloidal Iron Acid Mucin Stain, Acid Mucopolysaccharides Stain
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