| REF No |
2435 |
| Purpose |
Designed for general laboratory use. It is the counting stain of the Gram staining method. This staining procedure allows microorganisms to be divided into two groups. |
| Ingredients |
Iodine, potassium iodide, 96% ethanol and PVP. |
| Working Principle |
Designed for general laboratory use. Lugol's solution is a type of iodine solution. This solution is used to fix the crystal violet dye added in the previous step and the complexes formed in the bacterial cells. Lugol's solution forms the crystal violet-iodine complex, allowing it to bind more firmly to the bacterial cell wall. This staining procedure allows microorganisms to be divided into two groups. |
| Warnings, Precautions, Recommendations |
The products are for professional use only. Wear protective gloves/ protective clothing/ eye protection/ face protection. Not classified as hazardous according to the Regulation on the SEA of Substances or Mixtures (OG 11.12.2013 No: 28848 bis) and Directive 1272&2008 [CLP/GHS](EC). |
| Application
|
The smear is usually fixed by heating.
If the smear is too acidic, cover with Sodium Hydrogen Carbonate Solution for 15-30 seconds.
Cover the smear with Crystal Violet Stain (GBL-2434) for 1-2 minutes.
Wash with distilled water.
Cover the smear with Gram Lugol's Solution (GBL-2435) for 1 minute.
Wash with distilled water.
Cover smear with Denatured Alcohol (GBL-2891) for 5-15 seconds.
Wash with distilled water.
Cover the smear with Safranin Stain (GBL-2444) or Gram Fuchsin Aqueous Stain (GBL-2424) for 1 minute.
Gently washed with distilled water. Air dried. Examined. |
| Theoretical Results |
|
Gram positive bacteria |
Blue-purple |
|
Gram negative bacteria |
Pink-red |
|
| Related Media
|
|
| Shelf Life |
24 months |
| Shelf Life After Opening the Lid |
6 months |
| Storage Temperature |
15-25°C |
| pH (at 25°C) |
|
| Transportation Information
|
It is not classified as a dangerous product according to transport rules. |
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Parcel |
|
442435050100 |
Bottle |
100 ml |
100 ml, HDPE White Bottle with Dropper |
72 |
|
442435050250 |
Bottle |
250 ml |
250 ml, PP White Bottle with Dropper |
32 |
|
442435050500 |
Bottle |
500 ml |
500 ml, HDPE White Bottle |
24 |
|
442435011000 |
Bottle |
1000 ml |
1000 ml, HDPE Brown Bottle |
20 |
|
| Keywords
|
Gram-negative bacteria, Gram-positive bacteria, differentiation of bacteria, identification of bacterial isolates, staining technique in microbiology, Gram’s crystal violet Solution, Gram’s iodine Solution, Gram’s Decolorizer Solution, Gram’s safranin Solution, Gram staining, Gram's method, lugol. |