| REF No |
5152 |
| Purpose |
It is used in the examination of tissue sections, especially lymphoid and hematopoietic systems and other cells and tissues, as well as microorganisms, fungi and parasites, by light microscopy. |
| Ingredients |
A) Methanolic Fixative: Methanol
B) May-Grünwald Stain Solution: Glycerol, Magnesium acetate, Calcium acetate, Azure II, Methylene blue, Eosin Y, Lithium chloride, Potassium chloride
C) Giemsa Solution: Giemsa stain, Glycerol, Lithium chloride, Potassium chloride, Magnesium acetate, Calcium acetate
D) Buffer Solution. Tris buffer
E) Acetic Acid Solution: Glacial acetic acid. |
| Working Principle |
This staining method is one of the most commonly used neutral staining methods. In the neutral staining method aimed at by this kit, two neutral dye solutions are used together. Giemsa and May-Grünwald dye solutions have two basic characteristics: acidic (anionic) and basic (cationic). These dyes are a mixture of xanthene and thiazine group dyes.
Unlike the staining method used in smears, it includes a washing step with acetic acid solution in order to increase the discrimination. At the same time, the Giemsa dye used in the kit contains much less concentration of glycerol. |
| Warnings, Precautions, Recommendations |
Danger. Highly flammable liquid and vapour. Causes severe skin burns and eye damage. Toxic if swallowed, in contact with skin or if inhaled. Causes damage to organs. IF SWALLOWED: Rinse mouth. DO NOT induce vomiting. Keep away from heat/sparks/flames/hot surfaces. - No smoking. Wear protective gloves/shields/eye protection/face protection. IF ON SKIN (or hair): Remove/take off immediately all contaminated clothing. Rinse skin with water/shower. IF INHALED: Remove victim to fresh air and keep in a position comfortable for breathing easily. Call the NATIONAL POISON CONSULTATION CENTER ON 114 or a doctor/physician. In case of fire: Use dry powder or dry sand for extinguishing. Store in a well-ventilated area. Keep locked up. Keep cold. |
| Application
|
1. Immerse the tissue section in A- Methanolic Fixative reagent. After 5 minutes, shake it off.
2. Cover the tissue section with B- May-Grünwald Staining Solution reagent. Wait 3 minutes.
3. Without pouring the staining solution from the preparation, add the same amount of Buffer Solution (*). Mix by blowing or small agitations. After 3 minutes, shake it off.
4. Place the tissue section in the container of Ready Giemsa Solution (*). After 10 minutes, shake it off.
5. Place the tissue section in the container of Acid Solution (*). After 3-5 seconds, shake it off.
6. Rinse briefly with distilled water.
7. Dehydrate, clean, cover with xylene-based covering medium and examine under a microscope. |
| Theoretical Results |
|
Bile pigments |
Green |
|
Collagen, muscle, bone |
Pale pink |
|
Microorganisms (including H. pylori) and fungi |
Violet blue to dark blue |
|
Parasites (including Rickettsiae) |
Reddish purple to violet blue |
|
Starch granules, cellulose |
Sky blue |
|
Pigments |
ellow to brown; green if dichromate fixatives are not used |
|
Nucleus |
Dark blue to purple |
|
Erythrocytes |
Light pink |
|
Cytoplasm |
Pinkish rose to pale dirty bluish |
|
Mast cells |
Violet blue |
|
| Related Media
|
|
| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
15-25 °C |
| pH (at 25°C) |
|
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
445152030250 |
Piece |
250 mL |
250 ml, PP White Bottle with Dropper |
1 kit |
|
445152020500 |
Piece |
500 mL |
500 ml, HDPE White Bottle 28 mm |
1 kit |
|
| Keywords
|
May Grünwald Giemsa |