| REF No |
5025 |
| Purpose |
It is used for light microscopic examination of blood cells, lymphohemopoietic tissues and parasites in tissue sections. |
| Ingredients |
Glycerol, Methylene blue, Eosin Y, Methanol, Giemsa stain, Buffers. |
| Working Principle |
May Grunwald-Giemsa stain (MGG) is a Romanowsky stain routinely used for staining air-dried cytological smears, blood and bone marrow smears. Cytological preparations from FNAC and serous fluids are normally processed with May Grunwald-Giemsa stain. It is useful for examining cellular morphology and is superior to PAP staining for examining the cytoplasm, granules, vacuoles and basement membrane.
The working principle of MGG stain is the same as other Romanowksy stains. Polychromatic Romanowksy stains contain different proportions of methylene blue (and reagent-related thiazine dyes such as azure B) as the cation (+ highly charged, basic) component and eosin Y as the anion (- highly charged, acidic) component. . The combination of cation and anion components produces the well-known Romanowsky effect or metachromasia.
The solvent methanol initially stabilises the cells. The basic stains carry net positive charges; consequently they stain nuclei (due to the negative charges of the phosphate groups of DNA and RNA molecules), basophil granulocyte granules and RNA molecules of the cytoplasm. Eosin carries a net negative charge and stains red blood cells and eosinophil granulocyte granules. |
| Warnings, Precautions, Recommendations |
Highly flammable liquid and vapour. Toxic if swallowed, in contact with skin or inhaled. Causes damage to organs. Keep away from heat/sparks/flame/hot surfaces. - No smoking. Wear protective gloves/protective clothing/eye protection/face protection. IF IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Rinse skin with water/shower. In case of exposure: Call NATIONAL POISON ADVICE CENTRE TELEPHONE 114 or doctor/physician |
| Application
|
Application of the Staining Method:
1. Cover the preparation with 10 drops of “A-May-Grünwald solution”. After 3 minutes go to the next step without pouring the reagent.
2. Add 10 drops of “Buffer working solution” to the reagent in the preparation. Mix the two reagents by blowing or small oscillations. After 6 minutes, shake the slide to remove the reagent.
3. Immerse the preparation in the container of “Buffer working solution”. After 1 minute, remove the slide and shake off the reagent.
4. Immerse the preparation in a beaker of “Giemsa working solution”. After 20 minutes, remove the slide and shake off the reagent.
5. Air dry the smear and examine under a microscope with an immersion objective. Dehydrate the tissue section, clean, cover with synthetic resin and examine under a microscope. |
| Theoretical Results |
|
Erythrocytes |
Reddish |
|
Platelets granules |
Violet purple |
|
Plasmodium chromatin |
Red |
|
Leukocyte nuclei |
Reddish purple |
|
Neutrophil granules |
Light purplish |
|
Basophil granules |
Dark purple or blackish |
|
Eosinophil granules |
Red to purple |
|
Monocyte cytoplasm |
Pale blue |
|
Plasmodium cytoplasm |
Blue |
|
Eosinophil cytoplasm |
Blue |
|
Lymphocyte cytoplasm |
Sky blue |
|
Neutrophil cytoplasm |
Shaded dirty pinkish |
|
| Related Media
|
|
| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
15-25°C |
| pH (at 25°C) |
|
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
Quantity in a Parcel |
|
445025050050 |
Piece |
50 mL |
50 ml, White Bottle with HDPE Dropper |
1 kit |
20 kits |
|
445025020250 |
Piece |
250 mL |
250 ml, PP White Bottle with Dropper |
1 kit |
15 kits |
|
445025020500 |
Piece |
500 mL |
500 ml, HDPE White Bottle 28 mm |
1 kit |
6 kits |
|
| Keywords
|
MGG, Giemsa Stain, May-Grunwald Giemsa Stain |