CONVASTAIN

2408-Giemsa Stain; Concentrate

Title Description
REF No 2408
Purpose It is usually used for staining blood cells.
Ingredients Giemsa stain, Methanol and Glycerol.
Working Principle Giemsa stain is a differential stain and contains a mixture of azure, methylene blue and eosin stain. It is specific for the phosphate groups of DNA and binds itself where there is a high amount of adenine-thymine bonding. Azur and eosin are acidic dyes that variably stain the basic components of cells such as cytoplasm, granules, etc. Methylene blue, on the other hand, acts as a basic stain for acidic components, especially the cell nucleus.
Warnings, Precautions, Recommendations Highly flammable liquid and vapour. Toxic if swallowed, in contact with skin or inhaled. Causes damage to organs.
Application Staining of Smears
1. Preparation of Dye Solution (Dilute Giemsa Solution):
▪ Take 5 ml of GBL-2408 "Giemsa Stain; Concentrate" into the staining container.
Add 45 ml of distilled water and mix well.
2. Staining with Giemsa Solution:
▪ Cover the preparation with methanol and fix for 5 minutes.
Immerse the preparation in the prepared staining solution.
After 15-30 minutes, remove the slide and shake to remove the reagent.
Wash with distilled water. Dry it.
Examine under a microscope.
Theoretical Results
Smear Staining Results:
Nucleus Red/purple
Lymphocytes Blue
Monocytes Pale blue
Neutrophilic granulocytes Granules light purple
Eosinophilic granulocytes Red/grey blue
Basophilic granulocytes Dark purple
Thrombocytes Purple
Erythrocytes Reddish
Blood parasites Nucleus light red
Staining Results of Paraffin Sections:
Cell nucleus, cells Blue, dark blue
Collagen, osteoid Pale blue
Eosinophilic granules Red
Acidophilic mucopolysaccharides Reddish purple
Acidophilic materials Orange red

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15/25°C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
442408050250 Bottle 250 ml 250 ml, PP White Bottle with Dropper 32
442408020500 Bottle 500 ml 500 ml, HDPE White Bottle 24
442408011000 Bottle 1000 ml 1000 ml, HDPE Brown Bottle 20

Keywords Giemsa stain, nucleic acid stain, blood film stain, Erythrocytes stain, lymphocyte cytoplasm stain, monocyte cytoplasm stain, leukocyte nuclear chromatin stain, peripheral blood smears
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