GBL® HEMA-ROSE

5061-Non-Specific Esterase Leukocyte Staining Kit

Title Description
REF No 5061
Purpose It is used to demonstrate alpha-naphthyl acetate esterase activity in leukocytes by light microscopy.
Ingredients A) Citrate solution pH: 5,4: Sodium chloride, Chloroform, Triton X-100
B) FB blue dye: Fast blue RR salt
C) Sodium nitrite solution: Sodium nitrite
D) Trismalate solution pH: 7.6: Tris, Malic acid, Tween 20
E) Alpha-Naphthyl acetate solution: Alpha-naphthyl acetate
F) Sodium fluoride solution: Sodium fluoride
G) Hematoxylin solution Gill-3: Ethylene Glycol, Hematoxylin, Sodium iodate,
Acetic acid
Working Principle Non-specific esterase leukocyte is an enzyme found in the cytoplasm of leukocytes (white blood cells). This enzyme hydrolyzes ester bonds, breaking down esters into their free acid and alcohol components. If leukocytes contain the enzyme esterase, the cells stain as black granulations.
Warnings, Precautions, Recommendations Harmful if swallowed, in contact with skin or inhaled. May be corrosive, irritating.
Application 1. Immerse the preparation in the “Fixative solution”. After 30 seconds, shake off the slide and remove the reagent.
2. Wash with distilled water for 1 minute.
3. Immerse the preparation in the “Incubation solution”. Incubate at 37 °C and protected from light. After 30 minutes, remove the slide and shake to remove the reagent.
4. Wash with distilled water for 2 minutes.
5. Stain the preparation with “G- Hematoxylin solution Gill-3” for 2 minutes. At the end of the time, shake the slide to remove the reagent.
6. Wash with distilled water for 15 seconds.
7. Air dry. Examine under a microscope. Use only aqueous covering medium if desired.
Theoretical Results
Alpha-Naphthyl acetate esterase activity Black granulation

Related Media
Shelf Life 1 year
Shelf Life After Opening the Lid
Storage Temperature 2-8 °C
pH (at 25°C) D) Trismalate solution pH: 7,6
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
445061050010 Piece 50 mL 50 ml, White Bottle with HDPE Dropper 1 kit

Keywords Non-Specific Esterase Stain, alpha-naphthyl acetate esterase
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