GBL® FERTI-ROSE

5005-Verheyen Spermatozoa Osmotic Tolerance Viability Test

Title Description
REF No 5005
Purpose Spermatozoids , hypoosmotic durability and their vitality detection can be done for is used .
Ingredients Hypoosmotic Earle's Solution: Eosin Y, Sodium Citrate, Glucose, Albumin bovine fraction V, Sodium chloride, Phosphate buffers.
Working Principle It is a semi-quantitative test based on the semi-permeability of the intact cell membrane, which causes the spermatozoa to swell. It takes place within living cells with intact membranes in a hypotonic solution. The Verheyen Spermatozoa Osmotic Tolerance Viability Test is a test that assesses the tolerance and viability of sperm cells to osmotic stress. This test is used to determine the ability of sperm cells to survive under different osmotic conditions.
Warnings, Precautions, Recommendations The products are for professional use only.
Application Hypoosmotic Tail Swelling Try it Fabrication :
1) Small One experiment to the tube 10 drop No: 1(Jeyendran Solution ) is added .
2) Another One experiment to the tube 10 drop No: 2( Hypoosmotic Earle's Solution ) is placed .
3) Another One experiment to the tube 10 drop bidistile This is placed .
3) all three into the tube 1'each drop' reserved (“ Front " Preparation " section see ) semen is deposited . It is mixed .
4) Tubes immediately 37 C degree This to the bathroom is placed .
5) 5- 10- 15- 20- 25-30 in minutes one each drop , both from the tube sample taken away in the microscope is examined . Besides 5- 15- in the 30'th minutes sample taken away examination can be done .
Try Liveliness Fabrication :
1) 5- 10- 15- 20- 25- 30( or 5- 15- 30) th in minutes 1 drop in the tube sample slide is taken . Over 1 drop No: 3(Eosin Y Dye ) is added . It is mixed quickly . in the microscope census makes .
Theoretical Results
Live cells Sperm tail swelling or curl with distinguish is done .
Dead cells Sperm tail not swell or not curl with distinguish is done .

Related Media
Shelf Life 2 year.
Shelf Life After Opening the Lid
Storage Temperature 2/8 °C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in Box Quantity per Carton
445005080001 Quantity 50 ml Vial 1.5 ml Glass; With Silicone PTFE Cover 1 set 20 set

Keywords Verheyen Spermatozoa Osmotic Tolerance Vitality Test, Verheyen Spermatozoa Osmotic Tolerance Vitality Test
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