GBL® ROSA-STAIN

2444-Gram Safranine Stain

Title Description
REF No 2444
Purpose Gram staining method is used to stain gram negative bacteria.
Ingredients Safranine, methanol, triton X-100, ethanol, sodium azide.
Working Principle Gram staining bacteria are divided into Gram positive and Gram negative. This distinction is based on structural differences in the bacterial membrane of the two groups, i.e. their ability to retain the dye. GBL® CONVASTAIN Gram Safranine Stain is used for contrast staining of bacterial species that do not retain their primary stain, i.e. Gram negative bacteria.
Warnings, Precautions, Recommendations Flammable liquid and vapour. Harmful if swallowed. May cause damage to organs.
Application 1)If the smear is too acidic, cover with "Sodium Hydrogen Carbonate Solution" for 15-30 seconds.
2)Cover the smear with "GBL-2434 Crystal Violet Dye" for 1-2 minutes.
3)Wash with distilled water.
4)Cover the smear with "GBL-2435 Lugol's Solution" for 1 minute.
5)Washed with distilled water.
6)Cover smear with "GBL-2891 Denatured Alcohol" for 5-15 seconds.
7)Washed with distilled water.
8)Cover the smear with "GBL-2444 Gram Safranine Stain" for 1 minute.
9)Gently washed with distilled water, air dried and examined under a microscope.
Theoretical Results
Gram positive bacteria Blue-black
Gram negative bacteria Red
Related Media
Shelf Life 24 months
Shelf Life After Opening the Lid 3 months
Storage Temperature 15-25°C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Parcel
442444050100 Bottle 100 ml 100 ml, HDPE White Bottle with Dropper 72
442444030250 Bottle 250 ml 250 ml, PP White Bottle with Dropper 32
442444020500 Bottle 500 ml 500 ml, HDPE White Bottle 24

Keywords Gram-negative bacteria, differentiation of bacteria, identification of bacterial isolates, staining technique in microbiology, Gram’s safranin Solution.
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