GBL® FERTI-ROSE KRUG

5038-Sperm Morphology Staining Kit; Kruger Method

Title Description
REF No 5038
Purpose GBL® FERTI-ROSE KRUG is a qualitative, non-automated diagnostic kit for professional use for the staining of human spermatozoa. The purpose of spermatozoa staining is to facilitate the differentiation between morphologically normal and abnormal spermatozoa and is intended to aid infertility evaluation.
Ingredients A) Fixative: Formaldehyde %37, Phosphate tamponu
B) Stain-I: Ethanol %96, Rose Bengal, Neutral red, Phosphate tamponu
C) Stain-II: Ethanol %96, Orange G, Pyronin Y, Phosphate tamponu
D) Stain-III: Janus green, Fast green FCF, Phosphate tamponu
Working Principle
Warnings, Precautions, Recommendations Danger Causes severe skin burns and eye damage. May cause respiratory irritation. Suspected to cause cancer. Do not breathe dust/fume/gas/mist/vapor/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Rinse skin with water/shower. IF INHALED: Remove the affected person to fresh air and keep in a comfortable position so that he/she can breathe easily. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. In case of exposure or interaction IF: Get medical advice/attention. If eye irritation persists: Seek medical advice/attention.

Translated with DeepL.com (free version)
Application 1. Place 5-10 µm of the semen sample, preferably liquefied (liquefied), on a slide. Spread a thin film on the slide.
2. Allow the smear sample to dry for approximately 10-15 minutes at 37°C or 20-30 minutes at room temperature.
3. Prepare the 'A-Fixative' container.
4. After the smear sample has dried, gently immerse it into the 'A-Fixative' container. Allow it to stabilize for 10-15 minutes.
5. At the end of the time, gently remove the preparation from the 'A-Fixative' container. Allow the excess to drain off by placing it upright on the absorbent paper. Do not wipe the sample!
6. Allow the preparation to dry at 37°C for about 10-15 minutes or at room temperature for about 20-30 minutes.
7. Gently immerse the preparation 1 time in the container with 'B- Stain I' and remove. Leave the preparation in 'B- Stain I' for 1 min. Then immerse the preparation 2 times in the container of distilled water and remove. Allow the excess water/stain to drain off.
8. Gently immerse the preparation 7 times in the container with 'C-Stain II' and remove. On the 7th immersion, leave the preparation in 'C-Stain II' for 5 minutes. Then immerse the preparation 2 times in the container of distilled water and remove. Allow the excess water/stain to drain off.
9. Gently immerse the preparation 7 times in the container with 'D-Stain III' and remove. At the 7th immersion, leave the preparation in 'D-Stain III' for 5 minutes. Then gently immerse the preparation 2 times in a container of distilled water and remove. Allow the excess water/stain to drain off.
10. Leave the preparation to dry in air and upright.
11. Examine under a microscope under 100x with a drop of immersion oil.
Theoretical Results
Acrosome Light green
Nucleus Red / Pink
Equatorial zone Pale Green
Center piece Dark Green
Tail Light Green

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15-25 °C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in Box
445038050050 Piece 50 mL 50 ml, HDPE White Bottle with Dropper 1 set

Keywords
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