GBL® ROSA-STAIN

5073-Rodanin Copper Staining Kit

Title Description
REF No 5073
Purpose Used to show copper deposits in tissue sections.
Ingredients A) Rhodanine Stock Dye: Rhodanine
B) Acetate Buffer: Sodium acetate buffer
C) Formalin Fixative: Sodium acetate, 37% Formaldehyde
D) Hematoxylin Dye, Gill-3: Hematoxylin, Acetic acid, Aluminum sulfate
Working Principle Rodanin Copper Staining Kit is a specialized histological staining method for the detection and visualization of copper deposits in tissues. Rhodanine staining provides specific staining of copper accumulated in tissues. Rhodanine reacts with copper deposited in tissues, causing it to stain specifically red-brown in color. This helps to highlight copper deposits under the microscope.
Warnings, Precautions, Recommendations Caution Danger Highly flammable liquid and vapor. Causes skin irritation. Causes serious eye irritation. Keep away from heat/sparks/flame/hot surfaces. - Do not smoke. IF IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Wash with plenty of soap and water. Rinse skin with water/shower. In case of skin irritation: Get medical advice/attention.
Application 1. Take the incubation container with 2 slide capacity from the set box. Mix the reagent bottles properly without adding the components. Add 16 drops of “A-Rhodanin Stock Dye”, 16 drops of “B-Acetate Buffer”, 16 drops of “C-Formalin Fixative” and 12 ml of distilled water. Close the lid and mix properly.
2. Place 2 preparations in the incubation dish. Incubate at 37 °C for 20 hours. At the end of the time, the dye in the preparation is removed by shaking on absorbent paper.
3. Wash with the ‘'Washing Solution’' described in the ‘'Preliminary Preparation’' section.
4.8 drops of "D-Hematoxylin Stain, Gill-3 Cover for 5 minutes. Then the preparation is shaken off on absorbent paper.
5. Wash with the “Washing Solution” described in the “Preliminary Preparation” section.
6. Dehydrated with alcohols. Cleaned with xylene. Covered with synthetic resin. Examined under a microscope.
Theoretical Results
Nucleus Blue
Copper Light brown to red orange

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 4-8 °C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
445073050050 Piece 50 mL 50 ml, White Bottle with HDPE Dropper 1 kit

Keywords Rhodanine Copper Staining Kit, Copper Staining, Histological Copper Stain
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