GBL® ROSA-STAIN

5103-Periodic Acid Schiff (PAS) Diastase Staining Kit

Title Description
REF No 5103
Purpose It is used for histologic visualization of glycogen, glycoprotein, mucoprotein and high molecular weight carbohydrates in tissue sections.
Ingredients A) Citric Acid Phosphate Buffer Solution: x8 Citric Acid, Phosphate Buffers
B) Diastase Enzyme: Diastase
C) Periodic Acid Solution: Periodic Acid
D) Schiff's Separator: Pararosaniline, Sodium metabisulfite, 37% Hydrochloric acid
E) Sodium Metabisulfite Solution: Sodium metabisulfite
F) Detection Solution: Sodium metabisulfite, 37% Hydrochloric acid
G) Mayer Hemalum Solution: Hematoxylin, Aluminum sulfate, Acetic acid, Glycerol
Working Principle Periodic acid selectively oxidizes 1,2-glycolic, primary amine, secondary amine and hydroxy ketonic groups. High molecular weight carbohydrates also carry these groups. As a result of oxidation, the ends of some carbon atoms in the structure become free. It retains leucofucsin dye (sulfurized fucsin dye) and prevents it from dissolving in water. The parts where the dye is retained appear magenta red in color. The enzyme diastase is used to remove glycogen from tissue sections. This allows glycogen and other mucoproteins to be distinguished and examined by light microscopy.

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Warnings, Precautions, Recommendations Danger Causes severe skin burns and eye damage. May cause cancer. Contact with acids releases oxide gas. Wear protective gloves/protective clothing/eye protection/face protection. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. In case of exposure or interaction IF: Get medical advice/attention.
Application 1.First prepare the “Diastase Incubation Solution”.
I.Add 35 ml distilled water and 5 ml A-Citric Acid Phosphate Buffer Solution x8 to a 40 ml container and mix.
II.Add 1 tube of B-Diastase Enzyme 6000U to this mixture and mix well.
III.Do not strain this mixture and use it fresh.
2.Immerse the preparation in a beaker of “Diastase Incubation Solution” and incubate for 30 minutes at room temperature.
3.Remove the slide from the incubation dish and shake to remove the reagent.
4.Wash with distilled water for 2 minutes.
5.Cover the tissue section with 10 drops of “C-Periodic Acid Solution” and remove the reagent by shaking the slide after 10 minutes.
6.Wash with distilled water for 2 minutes.
7.Cover with 10 drops of “D- Schiff's Separator” and shake the slide after 20 minutes to remove the reagent.
8.Wash with distilled water for 2 minutes.
9.Cover with 10 drops of “E- Sodium Metabisulfite Solution” and after 2 minutes shake the slide to remove the reagent.
10.Cover with 10 drops of “F- Fixing Solution” before washing with water. After 2 minutes, shake the slide to remove the reagent.
11.Wash with distilled water for 2 minutes.
12.Cover with 10 drops of “G- Mayer Hemalum Solution” and after 3 minutes shake the slide to remove the reagent.
13.Wash with distilled water for 5 minutes.
14.Dehydrate, cover with xylene-based synthetic resin and examine under a microscope.
Theoretical Results
Positive PAS Fields Magenta Red
Nucleus Blue

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 2-8 °C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in Box
445103050050 Piece 50 mL 50 ml, HDPE White Bottle with Dropper 1 set

Keywords Periodic Acid Schiff (PAS) Diastase Staining Kit, PAS
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