GBL® ROSA-STAIN

5020-Acid Resistant Staining Kit; Brilliant Green

Title Description
REF No 5020
Purpose It is used to detect acid-resistant bacteria by staining.
Ingredients A) Carbol Fuchsin Solution ZN: Fuchsin, Phenol, Ethanol 96%
B) Acid Alcohol Solution: Ethanol 96%, Methanol, Hydrochloric acid 37%
C) Brilliant Green Solution: Brilliant Green
Working Principle Carbol fuchsin solution is used to bind to the cell walls of acid-fast bacteria. After staining, the preparations are treated with an acidic alcohol solution. The acidic alcohol removes the carbol fuchsin dye from cells other than acid-fast bacteria. This step ensures that only acid-fast bacteria remain stained, leaving other cells or structures clear. After decolorization, the preparations are treated with brilliant green dye. This dye stains the background and other cells other than acid-fast bacteria green.
Warnings, Precautions, Recommendations Danger. Highly flammable liquid and vapor. Harmful if swallowed. Causes severe skin burns and eye damage. Suspected of causing genetic defects. Keep away from heat/sparks/flames/hot surfaces. Do not breathe dust/fume/gas/mist/vapors/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF SWALLOWED: If you feel unwell, call the NATIONAL POISON CONSULTATION CENTER ON 114 or a doctor/physician. IF ON SKIN (or hair): Remove/take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Application 1. The smear is stained with Carbol Fuchsin Stain by slowly heating the slide from the bottom using a burner or electric staining rack for 5 minutes until vaporization is observed. (Vaporization is continued for 3-5 minutes using low heat or intermittent heat.)
2. The slide is allowed to cool.
3. The slide is rinsed with water for 5-10 seconds.
4. The smear is covered with Acid Alcohol for 5-30 seconds.
5. It is washed very briefly with distilled water in a delicate manner.
6. The smear is stained with Brillant Green Stain for 1-2 minutes.
7. It is washed gently with distilled water.
8. It is dried in air.
9. The smear is examined with a 100 immersion objective.
Theoretical Results
Acid-resistant microorganisms Red
Others and background elements Green

Related Media
Shelf Life 1 year
Shelf Life After Opening the Lid
Storage Temperature 15/25°C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
445020020500 Piece 500 mL 500 ml, HDPE White Bottle 28 mm 1 kit

Keywords Acid-Fast Staining Kit; Brilliant Green
Acid-Fast Staining, Acid-resistant microorganisms stain
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