GBL® ROSA-STAIN

5023-PAS McManus Staining Kit

Title Description
REF No 5023
Purpose It is used to demonstrate glycogen, glycoprotein, mucoprotein and high molecular weight carbohydrates in tissue sections by light microscopy.
Ingredients A) Periodic Acid Solution: Periodic acid.
B) Schiff Reagent McManus: Pararosaniline, sodium metabisulfite, hydrochloric acid, activated charcoal.
C) Sodium Metabisulfite Solution: Sodium metabisulfite.
D) Detection Solution: Sodium metabisulfite, hydrochloric acid.
E) Mayer Hemalum Solution: 96% Ethanol, hematoxylin monohydrate, ammonium aluminum sulfate, sodium iodate, glycerol, triton X-100, citric acid monohydrate.
Working Principle
Warnings, Precautions, Recommendations
Application 1. Cover the tissue section with 10 drops of “A-Periodic Acid Solution”. After 10 minutes, shake the slide to remove the reagent.
2. Wash with distilled water for 2 minutes.
3. Cover with 10 drops of “B-Schiff Reagent McManus”. After 20 minutes, shake the slide to remove the reagent.
4. Wash with distilled water for 2 minutes.
5. Cover with 10 drops of “C-Sodium Metabisulfite Solution”. After 2 minutes, shake the slide to remove the reagent.
6. Cover with 10 drops of “D-Detection Solution” without rinsing with water. After 2 minutes, shake the slide to remove the reagent.
7. Wash with distilled water for 2 minutes.
8. Cover with 10 drops of “E-Mayer Hemalum Solution”. After 3 minutes, shake the slide to remove the reagent.
9. Wash with distilled water for 5 minutes.
10. Dehydrate, clean, cover with xylene-based synthetic resin and examine under a microscope.
Theoretical Results
Positive PAS areas Magenta red
Nucleus Blue

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 2-8°C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
445023030050 Piece 50 mL 50 ml, White Bottle with HDPE Dropper 1 kit

Keywords PAS, McManus
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