| REF No |
2828 |
| Purpose |
It is used as a general-purpose nuclei stain, for the diagnosis of cytological cancers and cycles, and as a hematoxylin-eosin stain, which is the most widely used of the histological stains and stains different areas of tissue in different colors. |
| Ingredients |
Hematoxylin, Mercury oxide, Aluminum ammonium sulfate, Acetic acid, Ethylene glycol, Stabilizers. |
| Working Principle |
Eosin Y is an indispensable histologic staining technique in pathology laboratories, usually together with Hematoxylin. The principle is that hematoxylin, which gives a blue-violet color, and Eosin, which gives a pinkish red color, stain the cells.
Hematoxylin is a dye with an alkaline pH and stains the cell nucleus (nucleic acid) and calcium deposits. Eosin Y stains structures such as cytoplasm, protein and amyloid. Hematoxylin itself does not stain directly. Hematoxylin reacts with oxygen and oxidizes to its active form Hemathein and the staining becomes visible.
In Hematoxylin and Eosin Y staining, besides cells, intranuclear and intracytoplasmic inclusion bodies, some secretory granules, amyloids, precipitations such as calcium and lead become visible. |
| Warnings, Precautions, Recommendations |
Caution. Harmful if swallowed.
Causes damage to organs after prolonged or repeated exposure (kidney).
Do not breathe dust/fume/gas/mist/vapor/spray.
If you feel unwell, seek medical advice/attention.
IF SWALLOWED: If you feel unwell, call NATIONAL POISON ADVICE CENTER PHONE 114 or a doctor/physician. |
| Application
|
MODIFIED PAP (REGRESSIVE METHOD) *
1. Fixed in 95% ethanol for 15 minutes.
2. Immersed 10 times in tap water (HYDRATION)
3. Hematoxylin Harris; soak in Acetic Acid (GBL_2828) for 2 minutes.
4. Immersed 10 times in tap water.
5. Dip in acid alcohol (1% HCl) 1-2 times.
6. Immersed 10 times in tap water.
7. Immersed in 95% ethanol 10 times (DEHYDRATION) (The same process is repeated 2-3 times in separate bottles).
8. Papanicolaou OG 6 Stain (GBL_2822) is kept for 2 minutes.
9. Immerse 10 times in 95% ethanol. (Repeat 2-3 times in separate bottles)
10. Papanicolaou, EA 50 Stain (GBL_2826) or Papanicolaou, EA 65 Stain (non gynecological) (GBL_2825); EA-65 (gynecological) for 5 minutes.
11. Immerse in 95% ethanol 10 times (repeat the same process 2-3 times in separate bottles).
12. Drying in an oven.
13. Immersed 20 times in xylol.
14. Cover with Entellan (GBL_2517) before xylene dries.
*In the progressive method, Gill's hematoxylin is used instead of Harris and kept for 1 min. In the regressive method, nuclei are overstained and this excess is removed with diluted acid solution (1% HCl). In the progressive method, nuclei are stained to the desired intensity and kept in “blueing” solution (ammonium hydroxide, lithium carbonate or Scoot's tap water) for 1 min instead of HCl. |
| Theoretical Results |
|
Papanicolaou |
Seed brown to black, cytoplasm orange, pink, green |
|
Hematoxylin eosin |
Seed bluish black, cytoplasm pink to reddish. |
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| Related Media
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| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
15-25°C |
| pH (at 25°C) |
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| Transportation Information
|
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| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
442828020500 |
Piece |
500 mL |
500 ml, HDPE White Bottle 28 mm |
24 |
|
442828011000 |
Piece |
1000 mL |
1000 ml, HDPE White Bottle 28 mm |
12 |
|
| Keywords
|
Hematoxylin - Eosin Staining, HxE, Hematoxylin, Hematoxylin Harris; With Acetic Acid |