GBL® ROSA-STAIN

5036-Verhoeff-VanGieson Staining Kit; Elastic Fibrils

Title Description
REF No 5036
Purpose It is a special staining method used in histology and pathology laboratories. This technique is used to highlight elastic fibers and collagen fibers.
Ingredients A) Hematoxylin solution, Alcoholic: Hematoxylin, Ethanol
B) Ferric chloride solution: Iron (III) chloride
C) Weigert's iodine solution: Iodine, Potassium iodide
D) VanGieson's solution Acid fuchsin, Picric acid
Working Principle Elastic stains bind to elastin proteins, making the elastic fibers appear darker. This helps to highlight the elastic fibers in tissue sections. A mixture of picro acid and acidic fuchsin stains collagen fibers red and other connective tissue components yellow. This different staining allows collagen and elastic fibers to be easily distinguished from each other. Verhoeff-VanGieson Staining Set; Elastic Fibrils provides important information in textural and pathological examinations by clarifying elastic and collagen fibers. The appearance of elastic fibers in dark purple/black and collagen fibers in red allows these structures to be easily distinguished under a light microscope.
Warnings, Precautions, Recommendations Poisonous Explosive. Harmful. Irritant. Flammable. Highly flammable liquid and vapor. Keep away from heat/sparks/flame/hot surfaces. Wear protective gloves/protective clothing/eye protection/face protection.
Application 1. Rinse the preparations in distilled water.
2. Stain for 10 minutes with elastic staining working solution.
3. At the end of the staining time, wash the preparations with distilled water.
4. Differentiate with 2% Ferric chloride working solution.
5. Wash with tap water.
6. Check under the microscope. If differentiation is excessive, go back to the Elastic staining working solution step.
7. Wash the preparation with 95% ethyl alcohol to remove the iodine.
8. Wash with distilled water.
9. Stain with “D-Van Gieson solution” for 1-3 minutes.
10. Wash with 95% ethyl alcohol.
11. Dehydrate with xylene. Cover.
Theoretical Results
Elastic fibrils Blue-black granulation
Nucleus From blue to black
Collagen Red
Muscles and other structures Yellow

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15-25°C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
445036050050 Piece 50 mL 50 ml, White Bottle with HDPE Dropper 1 kit

Keywords Elastic Fiber Staining, Collagen Fiber Staining
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