GBL® ROSA-STAIN

2406-Reticulocyte Stain; New Methylene Blue

Title Description
REF No 2406
Purpose It is used in the examination and counting of reticulocytes by light microscopy.
Ingredients New methylene blue, Potassium oxalate, Sodium chloride, Azure A, Sodium citrate, Preservative
Working Principle The new methylene blue binds to reticulocyte cell membranes, enabling cell detection. In addition to staining and counting reticulocytes, it also enables the study of cell morphology and structure. This enables information about the developmental processes and maturation stages of blood cells.
Warnings, Precautions, Recommendations
Application 1Samples and Preparation:
Devices, tools and equipment required for staining: Light microscope, immersion objective or cover staining unit, pipette, blue pipette tip, 250-1000 µl automatic pipette, tube, tube spore, slide drying apparatus, laboratory clock, stopwatch, absorbent paper, slides, immersion oil (GBL-2305) and GBL® Reticulocyte Stain (GBL-2406).
Sample and preparation: Peripheral or venous blood. Various defibrination agents can be used.
Staining method: Staining by mixing in the tube.
10 drops = 0.5 ml; 5 drops = 0.25 ml.
2. Application of Staining Method:
Put 10 drops (0.5 ml) of “Reticulocyte Stain” (GBL-2406) in a small test tube.
Add 3 drops of blood on it. Mix. Let it sit at room temperature for 15 minutes.
Spread on a slide.
Dry in air. Examine under a microscope.
Theoretical Results
Reticulum in reticulocytes Dark blue

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15/25°C
pH (at 25°C) pH: 7,3
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
442406050050 Piece 50 mL 50 ml, White Bottle with HDPE Dropper 20

Keywords Reticulocyte Stain With New Methylene Blue, Reticulocyte Stain
Up