CONVASTAIN

2423-Ziehl Neelsen Carbol Fuchsin Stain

Title Description
REF No 2423
Purpose Designed for general laboratory use. For the Ziehl Neelsen acid-fast staining method. Divides microorganisms according to whether they are acid-resistant or not.
Ingredients Fuchsin, phenol, ethanol.
Working Principle Acid-resistant organisms such as Mycobacterium tuberculosis contain large amounts of lipid substances called mycolic acids in their cell walls. These acids are resistant to staining by ordinary methods such as Gram staining.
The reagents used in Ziehl-Neelsen staining are carbol fuchsin, acid alcohol and methylene blue. Acid-resistant bacilli (ARB) appear bright red after staining.
Warnings, Precautions, Recommendations Hazard . Highly flammable liquid and vapor. Harmful if swallowed. Causes severe skin burns and eye damage. Suspected of causing genetic damage. Keep away from heat/sparks/flame/hot surfaces - Do not smoke. Do not breathe dust/fume/gas/mist/vapor/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF INGESTED: If you feel unwell, call NATIONAL POISON ADVICE CENTER PHONE 114 or a doctor/physician. IF IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Rinse skin with water/shower.
IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if they are worn and easy to do so. Continue rinsing
Application 1. The smear is stained with Carbol Fuchsin Stain (GBL-2423) by heating from the bottom for 5 minutes.
2. Cooled. Wash with distilled water for 5-10 seconds.
3. Cover the smear with Acid Alcohol (GBL-2426) for 5-30 seconds.
4. Gently washed very briefly with distilled water.
5. The smear is stained with Methylene Blue Alkali Stain, Löffler (GBL-2420) for 1-2 minutes.
6. Wash gently with distilled water. Air-dried and analysed.
Theoretical Results
Acid-resistant microorganisms Bright Red
Others and back field elements Blue

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15/25 °C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
442423050250 Piece 250 mL 250 ml, PP White Bottle with Dropper 32
442423020500 Piece 500 mL 500 ml, HDPE White Bottle 28 mm 24

Keywords acid-fast stain, Ziehl-Neelsen stain bacteria, asidofilik, Ziehl Neelsen, asit-fast, carbol-fuchsin, aside dirençliler, ZN,
Up