| REF No |
2423 |
| Purpose |
Designed for general laboratory use. For the Ziehl Neelsen acid-fast staining method. Divides microorganisms according to whether they are acid-resistant or not. |
| Ingredients |
Fuchsin, phenol, ethanol. |
| Working Principle |
Acid-resistant organisms such as Mycobacterium tuberculosis contain large amounts of lipid substances called mycolic acids in their cell walls. These acids are resistant to staining by ordinary methods such as Gram staining.
The reagents used in Ziehl-Neelsen staining are carbol fuchsin, acid alcohol and methylene blue. Acid-resistant bacilli (ARB) appear bright red after staining. |
| Warnings, Precautions, Recommendations |
Highly flammable liquid and vapour. Harmful if swallowed. Causes severe skin burns and eye damage. Suspected of causing genetic damage. |
| Application
|
-The smear is stained with Carbol Fuchsin Stain (GBL-2423) by heating from the bottom for 5 minutes.
-Cooled. Wash with distilled water for 5-10 seconds.
-Cover the smear with Acid Alcohol (GBL-2426) for 5-30 seconds.
-Gently washed very briefly with distilled water.
-The smear is stained with Methylene Blue Alkali Stain, Löffler (GBL-2420) for 1-2 minutes.
-Wash gently with distilled water. Air-dried and analysed. |
| Theoretical Results |
|
Acid-resistant microorganisms |
Bright Red |
|
Others and back field elements |
Blue |
|
| Related Media
|
|
| Shelf Life |
24 months |
| Shelf Life After Opening the Lid |
Right away |
| Storage Temperature |
15/25 °C |
| pH (at 25°C) |
|
| Transportation Information
|
Carriage/shipment by road (ADR/RID) - UN1993, Methanol/Ethanol, 3, II
Carriage/shipment by sea (IMDG) - UN1993, Methanol/Ethanol, 3, II
Carriage/shipment by air (ICAO/IATA) - UN1993, Methanol/Ethanol, 3, II |
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Parcel |
|
442423050250 |
Bottle |
250 ml |
250 ml, PP White Bottle with Dropper |
32 |
|
442423020500 |
Bottle |
500 ml |
500 ml, HDPE White Bottle |
24 |
|
| Keywords
|
acid-fast stain, Ziehl-Neelsen stain bacteria, asidofilik, Ziehl Neelsen, asit-fast, carbol-fuchsin, aside dirençliler, ZN, |