| REF No |
2456 |
| Purpose |
Designed for general laboratory use. Designed for Fournier Kinyoun acid-fast staining method at room temperature. |
| Ingredients |
Fuchsin, ethanol, propanol, glycerol, dimethyl sulphoxide, phenol. |
| Working Principle |
Mycobacterium, nocardia and corynebacterium do not have taychoic acid in their walls. About 35% of the wall is composed of lipids and especially mycolic acid. It is hypothesised that this feature of the wall increases the resistance of the bacterial wall against acid and alcohol. They do not easily release the dye they have taken; that is, they retain the fuchsin dye. Since the wall of bacilli is resistant to acidic alcohol, the dye remains in the bacilli. |
| Warnings, Precautions, Recommendations |
Highly flammable liquid and vapour.
Harmful if swallowed.
Causes severe skin burns and eye damage.
Suspected of causing genetic damage. May cause cancer.
Keep away from heat/sparks/flame/hot surfaces. - No smoking. |
| Application
|
1)Cover the smear with Carbol fuchsin solution FK (GBL-2456). After 3 minutes, shake the slide to remove the reagent.
2)Wash with distilled water for 15 seconds.
3)Wash the smear with Acid alcohol solution (GBL-2426). After 5 seconds, shake the slide to remove the reagent.
4)Wash with distilled water for 15 seconds.
5)Cover the smear with Methylene blue solution (GBL-2420). After 15 seconds, shake the slide to remove the reagent.
6)Wash with distilled water for 15 seconds
7)Air dry, preferably on a heated plate. Examine under a microscope. |
| Theoretical Results |
|
Acid-resistant microorganisms |
Red |
|
Other microorganisms |
Blue |
|
Back fields |
Blue |
|
| Related Media
|
|
| Shelf Life |
24 months |
| Shelf Life After Opening the Lid |
6 months |
| Storage Temperature |
15-25 °C |
| pH (at 25°C) |
|
| Transportation Information
|
Carriage/shipment by road (ADR/RID) - UN1993, Ethanol, 3, II
Carriage/shipment by sea (IMDG) - UN1993, Ethanol, 3, II
Carriage/shipment by air (ICAO/IATA) - UN1993, Ethanol, 3, II |
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Parcel |
|
442456020500 |
Bottle |
500 ml |
500 ml, HDPE White Bottle |
24 |
|
| Keywords
|
acid-fast stain, Ziehl-Neelsen stain bacteria, asidofilik, Ziehl Neelsen, asit-fast, carbol-fuksin, aside dirençliler, ZN, |