GBL® ROSA-STAIN

2405-Reticulocyte Stain; Brilliant Cresyl Blue

Title Description
REF No 2405
Purpose It is used in the examination and counting of reticulocytes by light microscopy.
Ingredients Brilliant cresyl blue, Sodium oxalate, Sodium chloride, Preservative
Working Principle As reticulocytes continue to mature in the bone marrow, they still retain RNA within the cell. For this reason, reticulocyte staining usually contains a dye that stains RNA.
Brilliant Cresyl Blue is a type of dye that is often used for reticulocyte staining. This dye is specifically formulated to stain RNA within cells. When reticulocytes are stained with Brilliant Cresyl Blue, the RNA within the cell is clearly colored and the reticulocytes are easily recognizable.
In this way, reticulocyte staining makes it easier to visualize and count young red blood cells under a microscope.
Warnings, Precautions, Recommendations
Application 1. Put 10 drops (0.5 ml) of “Reticulocyte Stain; Brilliant Cresyl Blue” (GBL-2405) in a small test tube.
2. Add 3 drops of blood. Mix. Let stand at room temperature for 15 minutes.
3. Spread on slide.
4. Dry in air.
5. Examine under microscope.
Theoretical Results
Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15/25°C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
442405050050 Piece 50 mL 50 ml, White Bottle with HDPE Dropper 72
442405020500 Piece 500 mL 500 ml, HDPE White Bottle 28 mm 24

Keywords Reticulocyte Stain Krezil Blue, Reticulocyte Stain
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