GBL® ROSA-STAIN

2118-Auramine Rhodamine Solution

Title Description
REF No 2118
Purpose It is used in microbiology and clinical diagnostics, especially for the detection of acid-resistant bacteria (ARB) such as Mycobacterium tuberculosis. The purpose of this solution is to allow such bacteria to accumulate in the cell walls and to be seen brightly under a fluorescence microscope
Ingredients Auramine o, Rhodamine B, Glycerol, Phenol, Propanol
Working Principle It is a staining technique used to visualize acid-fast bacilli using fluorescence microscopy. Acid-fast organisms exhibit a reddish yellow fluorescence color. Although the auramine-rodamine stain is not as specific as the Ziehl-Neelsen stain for acid-fast organisms (e.g. Mycobacterium tuberculosis or Nocardia), it gives faster results, so is often used as a screening tool.
Warnings, Precautions, Recommendations Danger. May cause severe skin burns and eye damage. Suspicion of genetic damage may occur. Use personal protective equipment. Wear personal protective equipment. If swallowed: Rinse mouth. DO NOT attempt to void.
IF IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Rinse skin with water/shower. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. Keep under lock and key.
Application 1. Cover with “A- Auramine Rhodamine Solution”. Shake off after 15 minutes.
2. Wash with distilled water.
3. Cover with “B- Acidic Bleaching Solution”. Shake off after 1 minute. You can change the reagent many times during this time.
4. Wash with distilled water.
5. Cover with “C- Potassium Permanganate Solution”. After 3 minutes, shake the preparation to remove the reagent.
6. Wash with running distilled water.
7. Examine under a microscope.
Theoretical Results
Acid resistant microorganisms Yellow to reddish fluorescence
Back areas Black

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15/25°C
pH (at 25°C)
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
442118020500 Piece 500 mL 500 ml, HDPE White Bottle 28 mm 24

Keywords Auramine Rhodamine Solution, ARB, Acid resistant microorganisms
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