GBL® ROSA-STAIN

2090-Crystal Violet Solution

Title Description
REF No 2090
Purpose With this staining procedure, microorganisms are separated as gram positive and gram negative.
Ingredients Crystal violet, ethanol, ammonium oxalate, dimethyl sulphoxide.
Working Principle Gram Positive/Negative status is a feature of the cell wall (peptidoglycan) structure of bacteria. The peptidoglycan structure of Gram Negative bacteria is thinner between the outer membrane and inner membrane than Gram Positive bacteria. In Gram positive bacteria, the thick peptidoglycan is capable of retaining crystal violet.
Warnings, Precautions, Recommendations Hazard Highly flammable liquid and vapour. Causes severe eye irritation. Suspected to cause cancer. Keep away from heat/sparks/flame/hot surfaces. Wear protective gloves/protective clothing/eye protection/face protection. IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Dry skin with water/shower. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. In case of exposure or interaction IF: Seek medical advice/attention. Keep under lock and key.
Application 1. Cover the smear with Crystal Violet Stain (GBL Stock Code-442090020500) for 5 minutes.
2. Wash gently with distilled water.
3. Cover the smear with Gram Lugol's solution (GBL Stock Code-442435020500) for 1 minute.
4. Gently washed with distilled water.
5. Cover the smear with Denatured Alcohol (Stock Code-442891020500) for 5-15 seconds.
6. Gently washed with distilled water.
7. Cover the smear with Gram Safranin Stain (GBL Stock Code-442444020500) or Gram Fuchsin Solution (GBL Stock Code-442424020500) for 1 minute.
8. Wash gently with distilled water.
9. Dry in air or in an oven and examine under a microscope.
Theoretical Results
Gram positive bacteria Blue-purple
Gram negative bacteria Pink-red

Related Media
Shelf Life 24 months
Shelf Life After Opening the Lid
Storage Temperature 15-25°C
pH (at 25°C) 5,09±0,3
Transportation Information Carriage/shipment by road (ADR/RID) - UN1993, Ethanol, 3, II
Carriage/shipment by sea (IMDG) - UN1993, Ethanol, 3, II
Carriage/shipment by air (ICAO/IATA) - UN1993, Ethanol, 3, II
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Parcel
442090020500 Adet 500 ml 500 ml, HDPE White Bottle 24
442090050250 Adet 250 ml 250 ml, PP White Bottle with Dropper 32
442090050100 Adet 100 ml 100 ml, HDPE White Bottle with Dropper 72

Keywords Crystal, Violet, purple.
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