GBL® FERTI-ROSE

2217-Eosin Nigrosin Sperm Stain

Title Description
REF No 2217
Purpose It is used to determine the number of live sperm when sperm motility is low.
Ingredients Sodium Chloride, Eosin Y, Nigrosin, Stabilizers.
Working Principle The basis of eosin nigrosin sperm staining is based on the principle that dead sperm take the dye in and appear stained because their membrane structure is disrupted.
Warnings, Precautions, Recommendations Caution. Causes serious eye irritation. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. If eye irritation persists: Seek medical advice/help. Wear protective gloves/protective clothing/eye protection/face protection.
Application 1. Mix the semen sample thoroughly.
2. Mix 0.05 ml of semen sample and 0.05 ml of Eosin-Nigrosin stain in a test tube and wait 30 seconds.
(1 drop = 0.05 ml)
3. After 30 seconds, prepare a smear preparation on a slide by taking 1 drop of the suspension and allow to air dry.
4. Fix in water-free fixative medium.
5. Examine under a bright-field microscope at x1000 magnification and immersion oil.
6. Count stained (dead) or unstained (live) cells with a laboratory cell counter.
Theoretical Results
Live spermatozoa Colorless
Dead spermatozoa Pink - Red

Related Media
Shelf Life 1 year.
Shelf Life After Opening the Lid
Storage Temperature 2/8 °C
pH (at 25°C) pH: 7,0±0,2
Transportation Information It is not classified as a hazardous product according to transportation rules.
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in Box
442217050050 Quantity 50 ml 50 ml, HDPE White Bottle with Dropper 1 Quantity

Keywords Eosin Nigrosin Sperm Stain
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