GBL® ROSA-STAIN

2826-Papanicolaou EA-50 Stain

Title Description
REF No 2826
Purpose Designed for general laboratory use. It is used for cytologic cancers and cycle diagnosis.
Ingredients Eosin Y, Phosphotungstic acid, Methanol, Ethylene glycol, Acetic acid, Light green SF
Working Principle Papanicolaou EA-50 Stain is a type of cellular examination stain. This stain is used in a screening test known as a Papanicolaou (Pap) smear test. The Pap smear test is a test for the early detection of cervical cancer and other reproductive diseases. EA-50 refers to a mixture of eosin-azur-eosin, a type of stain. This staining method is used to make cells better visible under a microscope. The structure and morphology of the cells are highlighted and examined with this stain. In this way, precancerous changes or other pathological conditions can be detected.
Warnings, Precautions, Recommendations Hazard Highly flammable liquid and vapor.
Toxic if swallowed, in contact with skin or inhaled.
Causes damage to organs.
Keep away from heat/sparks/flame/hot surfaces - No smoking.
Wear protective gloves/protective clothing/eye protection/face protection. IF IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Rinse skin with water/shower.
In case of exposure: Call NATIONAL POISON ADVICE CENTER TELEPHONE 114 or doctor/physician
Application Fixation
Vaginal smears are spread evenly on a slide. Air drying is usually not applied. Instead, it is fixed with 95% ethanol for 15-20 minutes.
1. Immersed 10 times in distilled water (HYDRATION)
2.Stain with 10 drops of “Hematoxylin Stain, Harris” (GBL-2828) for 2-5 minutes.
3.Immersed 10 times in distilled water.
4.Cover with 10 drops of “Hydrochloric Acid Solution; 0.5%” for 1 minute.
5.If step 4 has been done, the mite is washed with distilled water for a long time (up to 5 minutes).
6.Cover with 10 drops of 95% ethyl alcohol for 1 minute (DEHYDRATION)
7.10 drops of “Orange G Dye (OG-6)” (GBL-2822) for 1.5-3 minutes.
8.10 drops covered with 95% ethyl alcohol for 1 minute. (This process is repeated 2 times.)
9.10 drops are kept in “Polychrome Stain (EA-50)” (GBL-2826) for 1.5-3 minutes.
10.10 drops are covered with 95% ethyl alcohol for 1 minute.
11.Wait for drying.
12.After drying, it is immersed in xylene 20 times.
13.Cover with entallan / covering medium before xylene dries.
Theoretical Results
Nucleus From Brown to Black
Cytoplasm Orange, Pink, Green

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15-25°C
pH (at 25°C) pH: 5,3 ±0,05
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
442826020500 Piece 500 mL 500 ml, HDPE White Bottle 28 mm 24

Keywords Papanicolaou, Papanicolaou EA-50, EA-50, eosin, Pap smear, Cervical cancer screening, Papanicolaou method
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