| REF No |
5053 |
| Purpose |
It is used in the examination of acid mucopolysaccharides in tissue sections by light microscopy. |
| Ingredients |
A) Alcian Blue Stain pH; 2.5: Alcian blue, Glacial acetic acid, Thymol
B) Borate Buffer Solution: Boric acid, Di-Sodium tetraborate
C) Modified Mayer Hemalum Solution: 96% Ethanol, Hematoxylin, Glycerol, Citric acid |
| Working Principle |
Alcian Blue dye interacts with acidic mucopolysaccharides in a unique way. These mucopolysaccharides are found especially in mucosa and some other tissues. After the dye interacts with acidic mucopolysaccharides, it forms a complex with these compounds and this complex is blue. This blue color, which is concentrated in mucosal areas, provides the mucosa to be highlighted.
Alcian blue is a water-soluble polyvalent basic dye. The blue staining property comes from the copper in the molecule. In the acidic pH created by acetic acid,
a)sulfated acid mucopolysaccharides
b)carboxylated acid mucopolysaccharides
c)sulfated sialomucins (glycoproteins)
d)carboxylated sialomucins (glycoproteins) are stained with Alcian blue. |
| Warnings, Precautions, Recommendations |
Caution. Causes skin irritation. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN: Wash with plenty of soap and water. If skin irritation occurs: Get medical advice/attention. |
| Application
|
1. Cover the tissue section with 10 drops of “A- Alcian Blue pH:2.5 Solution”. After 30 minutes, shake the slide to remove the reagent.
2. Before washing with water, cover with 10 drops of “B- Borate Buffer Solution”. After 10 minutes, shake the slide to remove the reagent.
3. Wash with distilled water for 3 minutes.
4. Cover with 10 drops of “C- Modified Mayer Hemalum Solution”. After 5 minutes, shake the slide to remove the reagent.
5. Wash with distilled water for 2 minutes.
6. Dehydrate, clear, cover with xylene-based coverslip medium and examine under a microscope. |
| Theoretical Results |
|
Acid mucopolysaccharides |
Turquoise blue |
|
Nucleus |
Red |
|
| Related Media
|
|
| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
15-25 °C |
| pH (at 25°C) |
A) Alcian Blue Stain pH; 2.5:pH: 2,5 |
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
445053050050 |
Piece |
50 mL |
50 ml, White Bottle with HDPE Dropper |
1 kit |
|
| Keywords
|
Acidic mucins, carboxylated and sulfated mucosubstances, acid mucin staining, carboxylated mucin staining and sulfated mucous staining |