| REF No |
2777 |
| Purpose |
Sudan III stain is an oil dye and is commonly used in the diagnosis of oils and oil-containing substances. |
| Ingredients |
Sudan III, Gliserol, %96 Ethanol |
| Working Principle |
Sudan III stain is a fat stain and is commonly used in the diagnosis of fats and fat-containing substances.Sudan III stains fat drops with a red color, which helps to determine the presence of fats under a microscope. This is particularly useful for detecting fats in biological samples, for example in cell cultures or tissue sections.
Sudan III stain can also be used in some laboratory experiments. In particular, it is used to show the presence of lipids. Sudan III can be used to stain fatty acids, triglycerides and other types of fats.
One of the main uses of this stain is the identification of fat-containing samples in histology and microbiology. For example, it can be used to determine the presence of fat in tissue sections. Sudan III can also be used to analyze the fat content in foods.
Translated with DeepL.com (free version) |
| Warnings, Precautions, Recommendations |
Hazard Flammable liquid and vapor.
Keep away from heat/sparks/flame/hot surfaces. - No smoking.
Use only outdoors or in a well-ventilated area. IF IN CONTACT WITH SKIN (or hair): Immediately remove/remove all contaminated clothing. Rinse skin with water/shower. |
| Application
|
1. Freshly collected feces are made into a suspension.
2. Place 1 drop of fecal suspension on a slide.
3. Add 1 drop of 36% acetic acid solution and mix it with the help of a cotton pick.
4. Add 1 drop of Sudan III Dye (GBL-2777) and mix. Heat the slide over an open flame for a few seconds.
5. Close the coverslip while it is still hot and immediately examine it under the microscope. |
| Theoretical Results |
|
Dark orange colored particles with a diameter of 1-4 micrometers, which change with cooling. In normal feces, there are approximately 100 of these in one area. In cases of malabsorption, their diameter can increase greatly, reaching up to 75 micrometers, and their number also increases significantly. |
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| Related Media
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| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
15-25°C |
| pH (at 25°C) |
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| Transportation Information
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| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
442777050050 |
Piece |
50 mL |
50 ml, White Bottle with HDPE Dropper |
72 |
|
442777050100 |
Piece |
100 mL |
100 ml, PP White Bottle with Dropper |
48 |
|
442777050250 |
Piece |
250 mL |
250 ml, PP White Bottle with Dropper |
32 |
|
442777050500 |
Piece |
500 mL |
500 ml, HDPE White Bottle 28 mm |
24 |
|
| Keywords
|
Sudan III Stain, Lipid staining, Oil stain, Lipid staining, Oil detection |