GBL® ROSA-STAIN

5104-Modified ARB Staining Kit; FK

Title Description
REF No 5104
Purpose It is used to detect acid-resistant bacteria by staining.
Ingredients A) Carbol Fuchsin Solution FK: Fuchsin, Glycerol, Phenol, Ethanol 96%
B) Alcoholic Solution, 1% Sulfuric Acid: Methanol, Ethanol 96%, Sulfuric acid
C) Methylene Blue Solution: Methylene blue, Tween-80, Potassium buffer
Working Principle There is no taicoic acid in the walls of Mycobacterium, Nocardia, Cryptosporidium and Corynebacterium. Almost 35% of the wall consists of lipids and especially mycolic acid. It is assumed that this feature of the wall increases the resistance of the bacterial wall against acid and alcohol. They do not easily release the dye they take; in other words, they retain the Fuchsin dye. Since the wall of the bacilli is resistant to acidic alcohol, the dye remains in the bacilli.
Warnings, Precautions, Recommendations Danger. Highly flammable liquid and vapour. Harmful if swallowed. Causes severe skin burns and eye damage. Suspected of causing genetic defects. May cause cancer. Keep away from heat/sparks/flames/hot surfaces. - No smoking. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. P301+312: IF SWALLOWED: Call a POISON CENTER ON 114 or doctor/physician if you feel unwell. IF ON SKIN (or hair): Remove/take off immediately all contaminated clothing. Rinse skin with water/shower. IF exposed or concerned: Get medical advice/attention.
Application 1. Cover the preparation with “A-Carbol Fuchsin Solution FK”.
2. Heat from the bottom for less than 5 minutes.
3. Wash with distilled water for 15 seconds.
4. Cover the preparation with “B- Alcoholic Solution, 1% Sulfuric Acid”. After 15 seconds, shake the slide to remove the reagent.
5. Wash with distilled water for 15 seconds.
6. Cover the preparation with “C-Methylene Blue Solution”. After 30 seconds, shake the slide to remove the reagent.
7. Wash with distilled water for 15 seconds.
8. Dry in air, preferably on a heated table. Examine under a microscope.
Theoretical Results
Acid Resistant Microorganisms Red
Other Microorganisms Blue
Background Areas Blue

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15-25°C
pH (at 25°C) C) Methylene Blue Solution pH: 10.7 ±0.2
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
445104030250 Piece 250 mL 250 ml, PP White Bottle with Dropper 1 kit

Keywords ARB Staining, Acid-fast microorganism staining
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