GBL® ROSA-STAIN

5055-Acid Phosphatase Leukocyte Staining Kit

Title Description
REF No 5055
Purpose Used to examine acid phosphatase activity in blood, bone marrow or tissue samples.
Ingredients A) Citrate solution: Sodium chloride, Trisodium citrate, Condoms
B) FG-GBC red dye solution: Fast garnet GBC Sulfate, Stabilizer
C) Sodium nitrite solution: Sodium nitrite
D) Naphthol AS-BI phosphoric acid solution: Naphthol AS-BI phosphate
E) Acetate solution: Glacial acetic acid, Stabilizer, Condoms
F) Tartarate solution: L Tartaric acid, Stabilizer
G) Hematoxylin solution Gill-3: Hematoxylin, Glacial acetic acid, Aluminum sulfate, Stabilizer
Working Principle This method targets the enzyme acid phosphatase, which is found in the cell membranes of leukocytes. Acid phosphatase is an enzyme found on the inner surface of the cell membrane and is also present in leukocytes. The enzyme acid phosphatase hydrolyzes the phosphate molecules in the staining solution. As a result of this reaction, phosphate residues are formed in the cell membranes of cells. Naphthol AS, which is released by phosphatase activity in acidic medium, forms a colored complex as a result of diazolation with fast garnet GBC dyestuff.

Translated with DeepL.com (free version)
Warnings, Precautions, Recommendations
Application 1.Immerse the preparation in a container of “Fixative solution” brought to room temperature. After 30 seconds, shake the slide to remove the reagent. Do not allow the preparation to dry.
2.Wash with distilled water for 1 minute. Dry briefly (less than 15 seconds) or remove wetness with absorbent paper.
3.Immerse the preparations in containers with “AP incubation solution” and “TRAP incubation solution”. Incubate at 18-26 degrees C for 60 minutes, protected from light. Shake the slides out at the end of the time.
4.Wash with distilled water for 2 minutes. Dry briefly (less than 15 seconds) or remove wetness with absorbent paper.
5.Cover the preparation with 10 drops of “G- Hematoxylin solution Gill-3”. After 2 minutes, shake the slide to remove the reagent.
6.Wash several times with distilled water.
7.Air dry.
Theoretical Results
Acid phosphatase (AP) and tartarate-resistant acid
phosphatase (TRAP) activity

Dark red

Related Media
Shelf Life 1 year
Shelf Life After Opening the Lid
Storage Temperature 2-8 °C
pH (at 25°C) Component A pH: 5,4
Component D pH: 5,2
Komponent E pH: 5,2
Komponent F pH: 5,2
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in Box
445055050050 Piece 50 mL 50 ml, HDPE White Bottle with Dropper 1 set

Keywords Acid Phosphate Leukocyte, acid phosphatase activity, phosphatase (TRAP) activity
Up