GBL® ROSA-STAIN

2424-Gram Fuchsin Solution

Title Description
REF No 2424
Purpose It is the counting stain of the Gram staining method. With this staining procedure, microorganisms are separated as gram positive and gram negative.
Ingredients Fuchsin, phenol, ethanol.
Working Principle Gram Positive/Negative status is a feature of the cell wall (peptidoglycan) structure of bacteria. The peptidoglycan structure of Gram Negative bacteria is thinner between the outer membrane and inner membrane than Gram Positive bacteria. In Gram positive bacteria, the thick peptidoglycan is capable of retaining crystal violet. Lugol ensures the retention of the dye. Cell wall permeability of Gram positive bacteria is less. Therefore, they do not release dyes during decolourisation with denatured alcohol. In Gram-negative bacteria, alcohol dissolves the lipids in the outer membrane, the pores open and the crystal violet disperses. Gram-negative bacteria are then stained with fuchsin added to the medium.
Warnings, Precautions, Recommendations Hazard Highly flammable liquid and vapor. Keep away from heat/sparks/flame/hot surfaces. - No smoking.
Keep container tightly closed. Wear protective gloves/protective clothing/eye protection/face protection.
IF IN CONTACT WITH SKIN (or) HAIR: Immediately remove all contaminated clothing. Rinse skin with water/shower.
Store in a well ventilated area. Keep cold. Dispose of contents/container in accordance with national regulations.
Application 1)If the smear is too acidic, cover with Sodium Hydrogen Carbonate Solution for 15-30 seconds.
2)Cover the smear with Crystal Violet Stain (GBL-2434) for 1-2 minutes.
3)Wash with distilled water.
4)Cover the smear with Gram Lugol's Solution (GBL-2435) for 1 minute.
5)Washed with distilled water.
6)Cover the smear with Denatured Alcohol (GBL-2891) for 5-15 seconds.
7)Washed with distilled water.
8)Cover the smear with Gram Fuchsin Aqueous Stain (GBL-2424) for 1 minute.
9)Gently washed with distilled water. Air dried and analysed.
Theoretical Results
Gram positive bacteria Blue-black
Gram negative bacteria Red

Related Media
Shelf Life 2 years
Shelf Life After Opening the Lid
Storage Temperature 15-25°C
pH (at 25°C) 5,6±0,3
Transportation Information
Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
442424020500 Bottle 500 mL 500 ml, HDPE White Bottle 24

Keywords Gram-negative bacteria, Gram-positive bacteria, differentiation of bacteria, identification of bacterial isolates, staining technique in microbiology, Gram’s crystal violet Solution, Gram’s iodine Solution, Gram’s Decolorizer Solution, Gram’s safranin Solution, Gram staining, Gram's method.
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