| REF No |
2465 |
| Purpose |
As a general tissue stain, it is used to differentiate nucleus and cytoplasm. It is used as a hematoxylin-eosin stain, which is the most widely used histological stain and stains different parts of the tissue in different colors. |
| Ingredients |
Eosin Y, Eosin B, Phloxin, 96% Ethanol, Methanol, Propanol, Glycerol, Acetic acid, Buffer, Stabilizer. |
| Working Principle |
Eosin Y is an indispensable histologic staining technique in pathology laboratories, usually together with Hematoxylin. The principle is that hematoxylin, which gives a blue-violet color, and Eosin, which gives a pinkish red color, stain the cells.
Hematoxylin is a dye with an alkaline pH and stains the cell nucleus (nucleic acid) and calcium deposits. Eosin Y stains structures such as cytoplasm, protein and amyloid. Hematoxylin itself does not stain directly. Hematoxylin reacts with oxygen and oxidizes to its active form Hemathein and the staining becomes visible.
In Hematoxylin and Eosin Y staining, besides cells, intranuclear and intracytoplasmic inclusion bodies, some secretory granules, amyloids, precipitations such as calcium and lead become visible. |
| Warnings, Precautions, Recommendations |
Hazard Highly flammable liquid and vapor.
Causes severe eye irritation
Harmful if swallowed.
Causes damage to organs
Keep away from heat/sparks/flame/hot surfaces - Do not smoke.
Do not breathe dust/fume/gas/mist/vapor/spray.
Wear protective gloves/protective clothing/eye protection/face protection.
In case of exposure or feeling unwell: Call the NATIONAL POISON ADVICE CENTER on PHONE 114 or a doctor/physician. |
| Application
|
Product Usage
Acid Alcohol Solution (1% Acid Alcohol Solution) -For Differential
-1 ml Concentrated Hydrochloric Acid
-100 ml 70% Ethyl Alcohol
Ammonia Water (0.2% Ammonia Water Solution - Bluing)
-1 ml Ammonium hydroxide concentrate
-1000 ml distilled water
METHOD OF APPLICATION
- Initial preparation of deparaffinization solutions and dehydrating solutions will facilitate the study.
- Slides are removed by thoroughly draining in distilled water.
- Immerse them in a dye cup filled with Hematoxylin Harris; Acetic Acid (GBL_2828). Leave for 4 minutes.
- Place the slides under tap water for 2 minutes to remove excess stain.
- Tissues are differentiated by immersing and removing the slides in Acid Alcohol solution.
- The slides are quickly placed under tap water and the differentiation is stopped.
- The slides are kept in diluted Ammonia water to blue the tissues.
- The slides are kept under tap water until the tissue color becomes clear blue.
- The slides stained with Hematoxylin Harris; Acetic Acid (GBL_2828) are immersed in Eosin Y Stain 0.5%; Alcohol (GBL_2465). Leave for 1 minute.
- The staining process is finished by washing the slides under tap water.
- The stained slides are dehydrated starting with low concentrations of 70%, 95% and 100% alcohol and allowed to dry in an oven. |
| Theoretical Results |
|
Nucleus |
Blue |
|
Cytoplasm |
Pink |
|
Erythrocyte |
Terra red |
|
Muscle |
Red |
|
Fibrous Tissue |
Light pink |
|
Cartilage |
Light to dark blue in color |
|
Mycose |
Bluish |
|
Calcified Tissue |
Dark blue |
|
Protein Precipitates |
Pink |
|
Bacterial Communities |
Dark blue |
|
| Related Media
|
|
| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
15-25°C |
| pH (at 25°C) |
|
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
442465020500 |
Piece |
500 mL |
500 ml, PP White Bottle with Dropper |
24 |
|
442465011000 |
Piece |
1000 mL |
1000 ml, HDPE White Bottle 28 mm |
12 |
|
| Keywords
|
Eosin Y, Hematoxylin - Eosin Staining, HxE, Hematoxylin - Eosin Staining |