| REF No |
2826 |
| Purpose |
Designed for general laboratory use. It is used for cytologic cancers and cycle diagnosis. |
| Ingredients |
Eosin Y, Phosphotungstic acid, Methanol, Ethylene glycol, Acetic acid, Light green SF |
| Working Principle |
Papanicolaou EA-50 Stain is a type of cellular examination stain. This stain is used in a screening test known as a Papanicolaou (Pap) smear test. The Pap smear test is a test for the early detection of cervical cancer and other reproductive diseases. EA-50 refers to a mixture of eosin-azur-eosin, a type of stain. This staining method is used to make cells better visible under a microscope. The structure and morphology of the cells are highlighted and examined with this stain. In this way, precancerous changes or other pathological conditions can be detected. |
| Warnings, Precautions, Recommendations |
Hazard Highly flammable liquid and vapor.
Toxic if swallowed, in contact with skin or inhaled.
Causes damage to organs.
Keep away from heat/sparks/flame/hot surfaces - No smoking.
Wear protective gloves/protective clothing/eye protection/face protection. IF IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Rinse skin with water/shower.
In case of exposure: Call NATIONAL POISON ADVICE CENTER TELEPHONE 114 or doctor/physician |
| Application
|
Fixation
Vaginal smears are spread evenly on a slide. Air drying is usually not applied. Instead, it is fixed with 95% ethanol for 15-20 minutes.
1. Immersed 10 times in distilled water (HYDRATION)
2.Stain with 10 drops of “Hematoxylin Stain, Harris” (GBL-2828) for 2-5 minutes.
3.Immersed 10 times in distilled water.
4.Cover with 10 drops of “Hydrochloric Acid Solution; 0.5%” for 1 minute.
5.If step 4 has been done, the mite is washed with distilled water for a long time (up to 5 minutes).
6.Cover with 10 drops of 95% ethyl alcohol for 1 minute (DEHYDRATION)
7.10 drops of “Orange G Dye (OG-6)” (GBL-2822) for 1.5-3 minutes.
8.10 drops covered with 95% ethyl alcohol for 1 minute. (This process is repeated 2 times.)
9.10 drops are kept in “Polychrome Stain (EA-50)” (GBL-2826) for 1.5-3 minutes.
10.10 drops are covered with 95% ethyl alcohol for 1 minute.
11.Wait for drying.
12.After drying, it is immersed in xylene 20 times.
13.Cover with entallan / covering medium before xylene dries. |
| Theoretical Results |
|
Nucleus |
From Brown to Black |
|
Cytoplasm |
Orange, Pink, Green |
|
| Related Media
|
|
| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
15-25°C |
| pH (at 25°C) |
pH: 5,3 ±0,05 |
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
442826020500 |
Piece |
500 mL |
500 ml, HDPE White Bottle 28 mm |
24 |
|
| Keywords
|
Papanicolaou, Papanicolaou EA-50, EA-50, eosin, Pap smear, Cervical cancer screening, Papanicolaou method |