| REF No |
2444 |
| Purpose |
Gram staining method is used to stain gram negative bacteria. |
| Ingredients |
Safranine, methanol, triton X-100, ethanol, sodium azide. |
| Working Principle |
Gram staining bacteria are divided into Gram positive and Gram negative. This distinction is based on structural differences in the bacterial membrane of the two groups, i.e. their ability to retain the dye. GBL® CONVASTAIN Gram Safranine Stain is used for contrast staining of bacterial species that do not retain their primary stain, i.e. Gram negative bacteria. |
| Warnings, Precautions, Recommendations |
Flammable liquid and vapour. Harmful if swallowed. May cause damage to organs. |
| Application
|
1)If the smear is too acidic, cover with "Sodium Hydrogen Carbonate Solution" for 15-30 seconds.
2)Cover the smear with "GBL-2434 Crystal Violet Dye" for 1-2 minutes.
3)Wash with distilled water.
4)Cover the smear with "GBL-2435 Lugol's Solution" for 1 minute.
5)Washed with distilled water.
6)Cover smear with "GBL-2891 Denatured Alcohol" for 5-15 seconds.
7)Washed with distilled water.
8)Cover the smear with "GBL-2444 Gram Safranine Stain" for 1 minute.
9)Gently washed with distilled water, air dried and examined under a microscope. |
| Theoretical Results |
|
Gram positive bacteria |
Blue-black |
|
Gram negative bacteria |
Red |
|
| Related Media
|
|
| Shelf Life |
24 months |
| Shelf Life After Opening the Lid |
3 months |
| Storage Temperature |
15-25°C |
| pH (at 25°C) |
|
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Parcel |
|
442444050100 |
Bottle |
100 ml |
100 ml, HDPE White Bottle with Dropper |
72 |
|
442444030250 |
Bottle |
250 ml |
250 ml, PP White Bottle with Dropper |
32 |
|
442444020500 |
Bottle |
500 ml |
500 ml, HDPE White Bottle |
24 |
|
| Keywords
|
Gram-negative bacteria, differentiation of bacteria, identification of bacterial isolates, staining technique in microbiology, Gram’s safranin Solution. |