| REF No |
2408 |
| Purpose |
It is usually used for staining blood cells. |
| Ingredients |
Giemsa stain, Methanol and Glycerol. |
| Working Principle |
Giemsa stain is a differential stain and contains a mixture of azure, methylene blue and eosin stain. It is specific for the phosphate groups of DNA and binds itself where there is a high amount of adenine-thymine bonding. Azur and eosin are acidic dyes that variably stain the basic components of cells such as cytoplasm, granules, etc. Methylene blue, on the other hand, acts as a basic stain for acidic components, especially the cell nucleus. |
| Warnings, Precautions, Recommendations |
Highly flammable liquid and vapour. Toxic if swallowed, in contact with skin or inhaled. Causes damage to organs. |
| Application
|
Staining of Smears
1. Preparation of Dye Solution (Dilute Giemsa Solution):
▪ Take 5 ml of GBL-2408 "Giemsa Stain; Concentrate" into the staining container.
Add 45 ml of distilled water and mix well.
2. Staining with Giemsa Solution:
▪ Cover the preparation with methanol and fix for 5 minutes.
Immerse the preparation in the prepared staining solution.
After 15-30 minutes, remove the slide and shake to remove the reagent.
Wash with distilled water. Dry it.
Examine under a microscope. |
| Theoretical Results |
|
Smear Staining Results: |
|
Nucleus |
Red/purple |
|
Lymphocytes |
Blue |
|
Monocytes |
Pale blue |
|
Neutrophilic granulocytes |
Granules light purple |
|
Eosinophilic granulocytes |
Red/grey blue |
|
Basophilic granulocytes |
Dark purple |
|
Thrombocytes |
Purple |
|
Erythrocytes |
Reddish |
|
Blood parasites |
Nucleus light red |
|
|
|
|
Staining Results of Paraffin Sections: |
|
Cell nucleus, cells |
Blue, dark blue |
|
Collagen, osteoid |
Pale blue |
|
Eosinophilic granules |
Red |
|
Acidophilic mucopolysaccharides |
Reddish purple |
|
Acidophilic materials |
Orange red |
|
| Related Media
|
|
| Shelf Life |
24 months |
| Shelf Life After Opening the Lid |
3 months |
| Storage Temperature |
15/25°C |
| pH (at 25°C) |
|
| Transportation Information
|
Carriage/shipment by road (ADR/RID) - UN1993, Methanol, 3, II
Carriage/shipment by sea (IMDG) - UN1993, Methanol, 3, II
Carriage/shipment by air (ICAO/IATA) - UN1993, Methanol, 3, II |
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Parcel |
|
442408050250 |
Bottle |
250 ml |
250 ml, PP White Bottle with Dropper |
32 |
|
442408020500 |
Bottle |
500 ml |
500 ml, HDPE White Bottle |
24 |
|
442408011000 |
Bottle |
1000 ml |
1000 ml, HDPE Brown Bottle |
20 |
|
| Keywords
|
Giemsa stain, nucleic acid stain, blood film stain, Erythrocytes stain, lymphocyte cytoplasm stain, monocyte cytoplasm stain, leukocyte nuclear chromatin stain, peripheral blood smears |