| REF No |
5206 |
| Purpose |
It is used to show Gram (+) and Gram (-) bacteria by light microscopy. |
| Ingredients |
A- Gram Crystal Purple Solution: Crystal purple, ethanol, ammonium oxalate, dimethyl sulfoxide.
B- Gram Denatured Alcohol: Ethanol 96%, methanol, 2-propanol and stabilizer.
C- Gram Lugol Solution: Iodine, Potassium Iodide,96% Ethanol and PVP.
D- Gram Fuchsin Solution: Fuchsin, phenol and 96% ethanol. |
| Working Principle |
Gram-Positive/Negative status is a characteristic that depends on the cell wall (peptidoglycan) structure of bacteria. The peptidoglycan structure of Gram-Negative bacteria is thinner shaped between the outer membrane and the inner membrane than Gram-Positive bacteria. In gram-positive bacteria, thick peptidoglycan has the property of retaining crystal purple. Lugol ensures that the paint is retained. Gram-positive bacteria have less cell wall permeability. Therefore, they do not release dyes during decolorization with denatured alcohol. In gram-negative bacteria, alcohol dissolves lipids in the outer membrane, the pores open and the crystalline purple dissipates. In this way, Gram-negative bacteria are stained with fuchsin, which is then added to the medium. |
| Warnings, Precautions, Recommendations |
It is very flammable liquid and vapor. It leads to serious eye irritation. There is a suspicion of causing cancer. Keep away from heat/ sparks/flames/hot surfaces. Use protective gloves/ protective clothing/ eye protection/ face protection. In case of skin contact: Remove/Remove all contaminated clothing immediately. Dry your skin with water/ shower. In case of eye contact: Dry carefully with water for a few minutes. If it is attached and easy to do, remove the contact lenses. Keep rinsing. IF it is in case of exposure or interaction: Get medical help/care. Keep it under lock and key. |
| Application
|
1. Cover the preparation with “A-Gram Crystal Purple Solution”. After 5 minutes, shake up and pour.
2. It is washed off carefully with distilled water.
3. Cover the preparation with “C-Gram Lugol Solution”. After 1 minute, shake up and pour.
4. It is washed off carefully with distilled water.
5. Cover the preparation with “B-Gram Denatured Alcohol Solution”. After 5-15 seconds, shake up and remove the reagent.
6. It is washed off carefully with distilled water.
7. Cover the preparation with “D-Gram Fuchsin Solution”. After 1 minute, remove the reagent by shaking off the microscope slide.
8. It is washed off carefully with distilled water.
9. Dry in the air, preferably on a heated flat surface. Examine it under a microscope.
|
| Theoretical Results |
|
Gram positive bacteria |
Blue-purple |
|
Gram positive bacteria |
Pink-red |
|
| Related Media
|
|
| Shelf Life |
24 months. |
| Shelf Life After Opening the Lid |
6 months. |
| Storage Temperature |
15-25°C |
| pH (at 25°C) |
A- Gram Crystal Purple Solution : 5.0±0.3
B- Gram Denatured Alcohol : There are no suitable data.
C- Gram Lugol Solution : There are no suitable data.
D- Gram Fuchsin Solution : 5.6±0.3 |
| Transportation Information
|
Road transport/shipment (ADR/RID) - UN1993, Ethanol, 3, II
Transport/shipment by sea (IMDG) - UN1993, Ethanol, 3, II
Transport/shipment by air (ICAO/IATA) - UN1993, Ethanol, 3, II |
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
Quantity in a Parcel |
|
445206050050 |
Piece |
50 mL |
50 ml, White Bottle with HDPE Dropper |
1 kit |
20 kits |
|
445206050250 |
Piece |
250 mL |
250 ml, PP White Bottle with Dropper |
1 kit |
15 kits |
|
445206020500 |
Piece |
500 mL |
500 ml, HDPE White Bottle 28 mm |
1 kit |
6 kits |
|
| Keywords
|
gram jensen staining kit, positive bacteria, negative bacteria, microscopic, crystal purple stain, fucsin |