| REF No |
5010 |
| Purpose |
It is used to detect acid-resistant bacteria by staining. |
| Ingredients |
A) Carbol Fuchsin Solution: ZN Fuchsin, Ethanol 96%, Phenol
B) Acid Alcohol Solution: Methanol, Ethanol 96%, Sulfuric acid
C) Methylene Blue Solution: Methylene blue, Tween 80, Potassium buffer |
| Working Principle |
There is no taicoic acid in the walls of Mycobacterium, nocardia and corynebacterium. Almost 35% of the wall consists of lipids and especially mycolic acid. It is assumed that this feature of the wall increases the resistance of the bacterial wall against acid and alcohol. They do not easily release the dye they take; in other words, they retain the Fuchsin dye. Since the wall of the bacilli is resistant to acidic alcohol, the dye remains in the bacilli. |
| Warnings, Precautions, Recommendations |
Danger. Highly flammable liquid and vapour. Harmful if swallowed. Causes severe skin burns and eye damage. Suspected of causing genetic defects. May cause cancer. Keep away from heat/sparks/flames/hot surfaces. - No smoking. Use only outdoors or in a well-ventilated area. Wear protective gloves/protective clothing/eye protection/face protection. P301+312: IF SWALLOWED: Call a POISON CENTER ON 114 or doctor/physician if you feel unwell. IF ON SKIN (or hair): Remove/take off immediately all contaminated clothing. Rinse skin with water/shower. IF exposed or concerned: Get medical advice/attention. |
| Application
|
1. Cover the preparation with “A-Carbol Fuchsin Solution ZN”.
2. Heat from the bottom for less than 5 minutes.
3. Wash with distilled water for 15 seconds.
4. Cover the preparation with “B- Acid Alcohol Solution”. After 15 seconds, shake the slide and remove the reagent.
5. Wash with distilled water for 15 seconds.
6. Cover the preparation with “C-Methylene Blue Solution”. After 30 seconds, shake the slide and remove the reagent.
7. Wash with distilled water for 15 seconds.
8. Dry in air, preferably on a heated table. Examine under a microscope. |
| Theoretical Results |
|
Acid Resistant Microorganisms |
Red |
|
Other Microorganisms |
Blue |
|
Background Areas |
Blue |
|
| Related Media
|
|
| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
15-25°C |
| pH (at 25°C) |
C) Methylene Blue pH: 10.7± 0.2 |
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
445010020500 |
Piece |
500 mL |
500 ml, PP White Bottle with Dropper |
1 kit |
|
| Keywords
|
Modified ARB Staining Kit, Acid-Resistant bacteria, ZN |