| REF No |
5046 |
| Purpose |
It is used to examine the peroxidase activities of leukocytes. |
| Ingredients |
A) Benzidine solution: Ethanol 96%, Benzidine, Sodium nitroprusside
B) Hydrogen peroxide solution: Hydrogen peroxide, Acetanilide, Phosphate buffers
C) Hematoxylin Solution Gill-3: Hematoxylin, Sodium iodate, Ethylene glycol, Acetic acid |
| Working Principle |
Peroxidase Leukocyte Staining Set uses a suitable substrate to determine the activity of peroxidase enzymes. Peroxidase enzymes hydrolyze the substrate to form a colored product. This colored product allows peroxidase positive cells to be easily detected under the microscope. |
| Warnings, Precautions, Recommendations |
Caution. Danger. Harmful if swallowed. Causes serious eye irritation. Causes skin irritation. Highly flammable liquid and vapour. Do not breathe dust/fume/gas/mist/vapours/spray. May cause damage to organs through prolonged or repeated exposure. Wear protective gloves/protective clothing/eye protection/face protection. Causes serious eye irritation. Keep away from heat/sparks/flames/hot surfaces. - No smoking. Keep container tightly closed. IF ON SKIN (or hair): Remove/take off immediately all contaminated clothing. Dry skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if worn and easy to do. Continue rinsing. |
| Application
|
1. Cover the smear with 10 drops of A-Benzidine Solution. Wait 1 minute. Do not pour the reagent.
2. Add approximately 1-2 loopfuls (10-20 microliters) of B-Hydrogen Peroxide Solution to the first reagent. Mix by blowing and small agitations. After 1 minute, shake the slide to remove the reagent.
3. Wash in distilled water for 1 minute.
4. Cover the smear with 10 drops of C-Hematoxylin Solution Gill-3. After 1.5 minutes, shake the slide to remove the reagent.
5. Wash in distilled water for 30 seconds.
6. Dry in air. Examine under a microscope with an immersion lens. |
| Theoretical Results |
|
Peroxidase positive |
Granulation from gray to black |
|
Nucleus |
Blue |
|
| Related Media
|
|
| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
2-8 °C |
| pH (at 25°C) |
|
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
445046050050 |
Piece |
50 mL |
50 ml, White Bottle with HDPE Dropper |
1 kit |
|
| Keywords
|
Peroxidase Leukocyte Staining |