| REF No |
5039 |
| Purpose |
It is used to examine smears made from blood and bone marrow cells by staining. |
| Ingredients |
A) Wright-Giemsa Stain Solution: Wright dye, Giemsa, Glycerol, Methanol, Buffer
B) Wright-Giemsa Buffer Solution: Ethanol 96%, Phosphate Buffers
C) Wright-Giemsa Rinse Solution: Methanol, Brij 35 |
| Working Principle |
Wright-Giemsa staining can be applied quickly and effectively and helps to highlight a wide range of cellular structures. This staining method is one of the most widely used neutral staining methods. In the neutral staining method aimed at by this kit, a single dye solution is used. Wright-Giemsa dye solution has two basic characters: acidic (anionic) and basic (cationic). These dyes are a mixture of xanthene and thiazine group dyes. Xanthene-thiazine staining procedures are the most widely used and most modified staining procedures. This set in your hand is one of the newest modifications of this general method. Its advantages can be listed as follows:
1) Many dyes are contained in a single dye solution and have polychrome properties.
2) The number of stages is reduced to a minimum.
3) The staining time is greatly shortened.
4) Unlike its competitors with the same modification, the set contains organic buffers.
5) Wright-Giemsa stain solution contains organic buffers. The most important feature of the Wright staining procedure is its speed. Speed provides significant usage advantages over the Giemsa staining procedure. The Giemsa procedure offers the advantages of strong, high-quality staining and the immersion method. This method combines the significant advantages of both procedures. |
| Warnings, Precautions, Recommendations |
Highly flammable liquid and vapour. May cause respiratory irritation. Avoid breathing dust/fume/gas/mist/vapours/spray. Wear protective gloves/protective clothing/eye protection/face protection. |
| Application
|
1. Immerse the preparation in the container of A- Wright-Giemsa Stain Solution. After 3 minutes, shake it out.
2. Immerse the preparation in the container of B- Wright-Giemsa Buffer Solution. After 10-15 seconds, shake it out. Dip it in and out several times during this period.
3. Immerse the preparation in the container of C- Wright-Giemsa Rinse Solution. After 10-15 seconds, shake it out. Dip it in and out several times during this period.
4. Rinse with distilled water.
5. Dry in air.
6. Examine under a microscope using a immersion lens. |
| Theoretical Results |
|
Neutrophil cytoplasm |
Dusky pink |
|
Lymphocyte cytoplasm |
Sky blue |
|
Eosinophil cytoplasm |
Blue |
|
Plasmodium cytoplasm |
Blue |
|
Monocyte cytoplasm |
Pale blue |
|
Eosinophil granules |
Red to purple |
|
Basophil granules |
Dark purple or blackish |
|
Neutrophil granules |
Light purple |
|
Leukocyte nuclei |
Reddish purple |
|
Plasmodium chromatin |
Red |
|
Platelet granules |
Violet purple |
|
Erythrocytes |
Reddish |
|
| Related Media
|
|
| Shelf Life |
2 years |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
15/25°C |
| pH (at 25°C) |
A) Wright-Giemsa Stain Solution pH: 7.0±0.2 |
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
445039030050 |
Piece |
50 mL |
50 ml, White Bottle with HDPE Dropper |
1 kit |
|
| Keywords
|
Wright-Giemsa Staining |