| REF No |
5054 |
| Purpose |
It is used for medical diagnostic purposes in the detection of argentaffin structures in histological tissue samples. |
| Ingredients |
A) Periodic acid solution: Periodic acid
B) Silver nitrate solution: Silver nitrate
C) Methenamine borate: Methenamine, Sodium tetraborate
D) Gold chloride solution: Gold chloride
E) Sodium thiosulfate solution: Sodium thiosulfate, Preservative
F) Light green SF solution: Light green SF, Glacial acetic acid |
| Working Principle |
Gomori Methenamine Silver PASM Staining Kit is a staining kit used in histology to selectively highlight specific cell structures and tissue components, particularly basement membranes and fibrils. In this particular kit, the main components required to perform the Gomori silver Methenamine staining are Silver nitrate and Methenamine. Grocott Gomori's Methenamine silver is a special staining method more commonly used for fungi. The staining reaction is based on the fact that in the presence of periodic acid, the polysaccharides of the fungal cell wall are oxidized to aldehydes, which in turn reduce the nitrate-silver Methenamine complex due to the accumulation of reduced silver at the sites of the aldehydes, producing a brown to black color. |
| Warnings, Precautions, Recommendations |
Danger. Causes skin irritation. Causes serious eye irritation. May cause an allergic skin reaction. May damage fertility. May damage the unborn child. May cause damage to organs through prolonged or repeated exposure. Wear protective gloves/protective clothing/eye protection/face protection. Very toxic to aquatic life. Very toxic to aquatic life with long lasting effects. Obtain special instructions before use. Do not breathe mist or fumes. Avoid release to the environment. IF exposed or concerned: Get medical advice/attention. |
| Application
|
Silver nitrate/Methenamine borate working solution:
Dissolve 1 cup of “C- Methenamine borate” mixture in 30 ml of “B-Silver nitrate solution” at room temperature. Allow it to dissolve completely. The mixture should be prepared freshly. Only glassware and plastic tweezers etc. should be used.
The mixture reaction starts at 55-57 0C. Bring the mixture to 55-57 0C using a hot water bath. The mixture should be used immediately and discarded.
1. Wash the preparation with distilled water for 2 minutes.
2. Soak in “A- Periodic acid solution” for 10 minutes.
3. Soak in distilled water 3 times for 30 seconds.
4. Soak in freshly prepared Silver nitrate/methenamine borate working solution for 35-45 minutes.
5. Soak in distilled water 3 times for 30 seconds.
6. Soak in “D- Gold chloride solution” for 1 minute.
7. Soak in distilled water for approximately 30 seconds.
8. Soak in “E- Sodium thiosulfate solution” for 2 minutes.
8. Wash under running water for 3 minutes.
9. Soak in distilled water for approximately 30 seconds.
10. Soak in F- “Light green SF solution” for 2-3 minutes.
11. Soak in distilled water for 30 seconds.
12. Soak in 70% ethanol for 1 minute.
13. Soak in 96% ethanol for 1 minute.
14. Soak in 100% ethanol for 1 minute.
15. Soak in 100% ethanol for 1 minute.
16. Wash with xylene. |
| Theoretical Results |
|
Mushroom |
Dark brown to black |
|
Membranes |
Dark brown to black |
|
Background |
Green |
|
| Related Media
|
|
| Shelf Life |
1 year |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
2/8°C |
| pH (at 25°C) |
|
| Transportation Information
|
|
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
445054050050 |
Piece |
50 mL |
50 ml, White Bottle with HDPE Dropper |
1 kit |
|
| Keywords
|
Gomori silver methenamine staining, PASM |