| REF No |
5005 |
| Purpose |
Spermatozoids , hypoosmotic durability and their vitality detection can be done for is used . |
| Ingredients |
Hypoosmotic Earle's Solution: Eosin Y, Sodium Citrate, Glucose, Albumin bovine fraction V, Sodium chloride, Phosphate buffers. |
| Working Principle |
It is a semi-quantitative test based on the semi-permeability of the intact cell membrane, which causes the spermatozoa to swell. It takes place within living cells with intact membranes in a hypotonic solution. The Verheyen Spermatozoa Osmotic Tolerance Viability Test is a test that assesses the tolerance and viability of sperm cells to osmotic stress. This test is used to determine the ability of sperm cells to survive under different osmotic conditions. |
| Warnings, Precautions, Recommendations |
The products are for professional use only. |
| Application
|
Hypoosmotic Tail Swelling Try it Fabrication :
1) Small One experiment to the tube 10 drop No: 1(Jeyendran Solution ) is added .
2) Another One experiment to the tube 10 drop No: 2( Hypoosmotic Earle's Solution ) is placed .
3) Another One experiment to the tube 10 drop bidistile This is placed .
3) all three into the tube 1'each drop' reserved (“ Front " Preparation " section see ) semen is deposited . It is mixed .
4) Tubes immediately 37 C degree This to the bathroom is placed .
5) 5- 10- 15- 20- 25-30 in minutes one each drop , both from the tube sample taken away in the microscope is examined . Besides 5- 15- in the 30'th minutes sample taken away examination can be done .
Try Liveliness Fabrication :
1) 5- 10- 15- 20- 25- 30( or 5- 15- 30) th in minutes 1 drop in the tube sample slide is taken . Over 1 drop No: 3(Eosin Y Dye ) is added . It is mixed quickly . in the microscope census makes . |
| Theoretical Results |
|
Live cells |
Sperm tail swelling or curl with distinguish is done . |
|
Dead cells |
Sperm tail not swell or not curl with distinguish is done . |
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| Related Media
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| Shelf Life |
2 year. |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
2/8 °C |
| pH (at 25°C) |
|
| Transportation Information
|
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| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in Box |
Quantity per Carton |
|
445005080001 |
Quantity |
50 ml |
Vial 1.5 ml Glass; With Silicone PTFE Cover |
1 set |
20 set |
|
| Keywords
|
Verheyen Spermatozoa Osmotic Tolerance Vitality Test, Verheyen Spermatozoa Osmotic Tolerance Vitality Test |