| REF No |
2217 |
| Purpose |
It is used to determine the number of live sperm when sperm motility is low. |
| Ingredients |
Sodium Chloride, Eosin Y, Nigrosin, Stabilizers. |
| Working Principle |
The basis of eosin nigrosin sperm staining is based on the principle that dead sperm take the dye in and appear stained because their membrane structure is disrupted. |
| Warnings, Precautions, Recommendations |
Caution. Causes serious eye irritation. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. If eye irritation persists: Seek medical advice/help. Wear protective gloves/protective clothing/eye protection/face protection. |
| Application
|
1. Mix the semen sample thoroughly.
2. Mix 0.05 ml of semen sample and 0.05 ml of Eosin-Nigrosin stain in a test tube and wait 30 seconds.
(1 drop = 0.05 ml)
3. After 30 seconds, prepare a smear preparation on a slide by taking 1 drop of the suspension and allow to air dry.
4. Fix in water-free fixative medium.
5. Examine under a bright-field microscope at x1000 magnification and immersion oil.
6. Count stained (dead) or unstained (live) cells with a laboratory cell counter.
|
| Theoretical Results |
|
Live spermatozoa |
Colorless |
|
Dead spermatozoa |
Pink - Red |
|
| Related Media
|
|
| Shelf Life |
1 year. |
| Shelf Life After Opening the Lid |
|
| Storage Temperature |
2/8 °C |
| pH (at 25°C) |
pH: 7,0±0,2 |
| Transportation Information
|
It is not classified as a hazardous product according to transportation rules. |
| Ordering and Packaging Information |
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in Box |
|
442217050050 |
Quantity |
50 ml |
50 ml, HDPE White Bottle with Dropper |
1 Quantity |
|
| Keywords
|
Eosin Nigrosin Sperm Stain |